Fluorescent Dyes
Product#: BIOACT
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Fluorescent Dyes
Bioacts provides the full spectral range of reactive fluorescent dyes such as FSD FluorTM, FSD, Flamma® Fluors, ICG and other traditional dyes equipped with variety of reactive and functional groups along with fluorescent quenchers.
FSD Fluor™ |
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| FSD Fluor™ is a new generation of dye series with superb fluorescence intensity and high quantum yield comparing to traditional dyes. The fluorescence intensity after binding to biomolecules such as antibody, nucleotide, and protein maintains still excellent, FSD Fluor™ series is ideal for variety of biochemical and biological analytical applications with a less amount of dye conjugate. FSD Fluor™ series equipped with a variety of reactive groups and covers the full fluorescence spectral range from UV to NIR, hence the series is ideal for any applications in fluorescence spectroscopies and biological studies. With superior fluorophores and the wide spectral range, FSD Fluor™ dyes are suitable for every filter and are designed to meet the requirements for complex detection in the field of life science research. | ||||||
FSD Fluor™ NHS ester |
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| 488 | 555 | 594 | 647 | 680 | 750 | 800 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm | Spectrally similar dyes |
| KOSC1002 | FSD Fluor™ 488 | 495 | 519 | Alexa Fluor 488, FAM, Cy2 |
| KOSC1003 | FSD Fluor™ 555 | 554 | 565 | Alexa Fluor 555, DyLight 549, Cy3, ATTO 550 |
| KOSC1001 | FSD Fluor™ 594 | 591 | 617 | Alexa Fluor 594, DyLight 594 |
| KOSC1315 | FSD Fluor™ 647 | 651 | 667 | Alexa Fluor 647, Cy5, ATTO 647N, DyLight 650 |
| KOSC1515 | FSD Fluor™ 680 | 679 | 696 | Alexa Fluor 680, Cy5.5, DyLight 680, IRDye 680 |
| KOSC1702 | FSD Fluor™ 750 | 749 | 774 | Alexa Fluor 750, DyLight 755, Cy7, IRDye 750 |
| POSC1803 | FSD Fluor™ 800 | 774 | 790 | Cy7.5, DyLight 800, IRDye 800 |
Flamma® Fluors |
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| BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies. | |||||||||||||||||||||||||||||||||||||||||||||||||
Flamma® Fluors NHS ester |
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| 496 | 552 | 581 | 648 | 675 | 749 | 774 | |||||||||||||||||||||||||||||||||||||||||||
Flamma® Fluors Sulfo-NHS ester |
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| 496 | 552 | 581 | 648 | 675 | 749 | 774 | |||||||||||||||||||||||||||||||||||||||||||
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWSN1001 | Flamma® 496 Sulfo-NHS ester | 496 | 520 | Alexa488, FITC, Cy2 |
| PWSN1122 | Flamma® 552 Sulfo-NHS ester | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWSN1415 | Flamma® 581 Sulfo-NHS ester | 581 | 596 | Alexa594, DyLight594 |
| PWSN1215 | Flamma® 648 Sulfo-NHS ester | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWSN1515 | Flamma® 675Sulfo-NHS ester | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWSN1301 | Flamma® 749 Sulfo-NHS ester | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWSN1603 | Flamma® 774 Sulfo-NHS ester | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Flamma® Fluors Vinylsulfone |
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| 488 | 496 | 552 | 581 | 594 | 648 | 675 | 749 | 774 | 800 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWA1002 | Flamma® 488 Vinylsulfone | 495 | 519 | Alexa488, Cy2 |
| CWA1001 | Flamma® 496 Vinylsulfone | 496 | 520 | Alexa488, FAM |
| PWA1122 | Flamma® 552 Vinylsulfone | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550, CF555 |
| PWA1415 | Flamma® 581 Vinylsulfone | 581 | 596 | Alexa594, DyLight594 |
| KOA1001 | Flamma® 594 Vinylsulfone | 590 | 617 | Alexa594, DyLight594 |
| PWA1215 | Flamma® 648 Vinylsulfone | 648 | 683 | Alexa647, DyLight650, Cy5 |
| PWA1515 | Flamma® 675 Vinylsulfone | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWA1308 | Flamma® 749 Vinylsulfone | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWA1603 | Flamma® 774 Vinylsulfone | 774 | 795 | Cy7.5, CF 770, DyLight 800, IRDye800 |
| POA1803 | Flamma® 800 Vinylsulfone | 774 | 789 | Cy7.5, DyLight800, IRDye800 |
Flamma® Fluors Isothiocyanate |
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| 496 | 552 | 581 | 648 | 675 | 749 | 774 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWI1001 | Flamma® 496 Isothiocyanate | 494 | 520 | Alexa488, FITC, Cy2 |
| PWI1122 | Flamma® 552 Isothiocyanate | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWI1415 | Flamma® 581 Isothiocyanate | 578 | 593 | Alexa594, DyLight594 |
| KWI1215 | Flamma® 648 Isothiocyanate | 648 | 663 | Alexa647, DyLight650, Cy5 |
| KWI1515 | Flamma® 675 Isothiocyanate | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWI1308 | Flamma® 749 Isothiocyanate | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWI1603 | Flamma® 774 Isothiocyanate | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Flamma® Fluors Maleimide |
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| 496 | 552 | 581 | 648 | 675 | 749 | 774 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWM1001 | Flamma® 496 Maleimide | 496 | 520 | Alexa488, FAM |
| CWM1058 | Flamma® 552 Maleimide | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550, CF555 |
| KWM1415 | Flamma® 581 Maleimide | 581 | 596 | Alexa594, DyLight594 |
| KWM1042 | Flamma® 648 Maleimide | 648 | 663 | Alexa647, DyLight650, Cy5, ATTO 647N, CF647 |
| PWM1415 | Flamma® 675 Maleimide | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWM1215 | Flamma® 749 Maleimide | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWM1515 | Flamma® 774 Maleimide | 774 | 806 | Cy7.5, DyLight800, IRDye800 |
Flamma® Fluors Hydrazide |
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| 496 | 552 | 581 | 648 | 675 | 749 | 774 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWH1001 | Flamma® 496 Hydrazide | 496 | 520 | Alexa488, FITC, Cy2 |
| PWH1122 | Flamma® 552 Hydrazide | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWH1415 | Flamma® 581 Hydrazide | 578 | 593 | Alexa594, DyLight594 |
| PWH1215 | Flamma® 648 Hydrazide | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWH1515 | Flamma® 675 Hydrazide | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWH1301 | Flamma® 749 Hydrazide | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWH1603 | Flamma® 774 Hydrazide | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Flamma® Fluors Dichlorotriazine |
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| 552 | 581 | 648 | 675 | 749 | 774 |
| Quick link (Cat.#) | Series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWR2112 | Flamma® 552 Dichlorotriazine | 550 | 564 | Alexa555, DyLight550, Cy3, ATTO550 |
| KWR2415 | Flamma® 581 Dichlorotriazine | 578 | 593 | Alexa594, DyLight594 |
| PWR2215 | Flamma® 648 Dichlorotriazine | 648 | 667 | Alexa647, DyLight650, Cy5 |
| PWR2515 | Flamma® 675 Dichlorotriazine | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWR2301 | Flamma® 749 Dichlorotriazine | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWR2603 | Flamma® 774 Dichlorotriazine | 774 | 800 | DyLight800, Cy7.5, IRDye800, CF770 |
Flamma® Fluors Carboxylic acid |
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| 552 | 648 | 675 | 749 | 774 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWC1101 | Flamma® 552 Carboxylic acid | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWC1201 | Flamma® 648 Carboxylic acid | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWC1501 | Flamma® 675 Carboxylic acid | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWC1308 | Flamma® 749 Carboxylic acid | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWC1603 | Flamma® 774 Carboxylic acid | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Flamma® Fluors Amine |
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| 496 | 552 | 581 | 648 | 675 | 749 | 774 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWE1001 | Flamma® 496 Amine | 496 | 520 | Alexa488, FITC, Cy2 |
| PWE1122 | Flamma® 552 Amine | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWE1415 | Flamma® 581 Amine | 578 | 593 | Alexa594, DyLight594 |
| PWE1215 | Flamma® 648 Amine | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWE1515 | Flamma® 675 Amine | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWE1301 | Flamma® 749 Amine | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWE1603 | Flamma® 774 Amine | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Flamma® Fluors Thiol |
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| 496 | 552 | 581 | 648 | 675 | 749 | 774 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWT1001 | Flamma® 496 Thiol | 494 | 520 | Alexa488, FITC, Cy2 |
| CWT1058 | Flamma® 552 Thiol | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWT1415 | Flamma® 581 Thiol | 578 | 593 | Alexa594, DyLight594 |
| KWT1042 | Flamma® 648 Thiol | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWT1415 | Flamma® 675 Thiol | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWT1215 | Flamma® 749 Thiol | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWT1515 | Flamma® 774 Thiol | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Flamma® Fluors Alkyne |
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| 496 | 552 | 581 | 648 | 675 | 749 | 774 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWK1001 | Flamma® 496 Alkyne | 496 | 520 | Alexa488, FITC, Cy2 |
| PWK1122 | Flamma® 552 Alkyne | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWK1415 | Flamma® 581 Alkyne | 581 | 596 | Alexa594, DyLight594 |
| PWK1215 | Flamma® 648 Alkyne | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWK1515 | Flamma® 675 Alkyne | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWK1301 | Flamma® 749 Alkyne | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWK1603 | Flamma® 774 Alkyne | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Flamma® Fluors PEG4-Alkyne |
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| 496 | 552 | 581 | 648 | 675 | 749 | 774 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWG1001 | Flamma® 496 PEG4-Alkyne | 496 | 520 | Alexa488, FITC, Cy2 |
| PWG1122 | Flamma® 552 PEG4-Alkyne | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWG1415 | Flamma® 581 PEG4-Alkyne | 581 | 596 | Alexa594, DyLight594 |
| PWG1215 | Flamma® 648 PEG4-Alkyne | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWG1515 | Flamma® 675 PEG4-Alkyne | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWG1301 | Flamma® 749 PEG4-Alkyne | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWG1603 | Flamma® 774 PEG4-Alkyne | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Flamma® Fluors ADIBO |
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| 496 | 552 | 581 | 648 | 675 | 749 | 774 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| DWC1001 | Flamma® 496 ADIBO | 496 | 520 | Alexa488, FITC, Cy2 |
| DWC1011 | Flamma® 552 ADIBO | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| DWC1415 | Flamma® 581 ADIBO | 581 | 596 | Alexa594, DyLight594 |
| DWC1021 | Flamma® 648 ADIBO | 648 | 663 | Alexa647, DyLight650, Cy5 |
| DWC1051 | Flamma® 675 ADIBO | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| DWC1031 | Flamma® 749 ADIBO | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| DWC1061 | Flamma® 774 ADIBO | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Flamma® Fluors Azide |
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| 496 | 552 | 581 | 648 | 675 | 749 | 774 |
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWZ1001 | Flamma® 496 Azide | 496 | 520 | Alexa488, FITC, Cy2 |
| PWZ1122 | Flamma® 552 Azide | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWZ1415 | Flamma® 581 Azide | 581 | 596 | Alexa594, DyLight594 |
| PWZ1215 | Flamma® 648 Azide | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWZ1515 | Flamma® 675 Azide | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWZ1301 | Flamma® 749 Azide | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWZ1603 | Flamma® 774 Azide | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
ICG Dyes |
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| ICG is clinically approved NIR dye and used in medical diagnostics, in vitro, vivo and animal model study. NIR fluorescence allows to observe the deep image from the surface of skin and its usage spreads to a wide range of research fields. ICG fluorescence method is safe and cost effective technique and used in a wide range of medical fields such as breast cancer sentinel lymph node navigation, detecting cerebral vessels, coronary arteries and biliary trees, tracking tumor location, detecting small HCC, etc. The method has several advantages such as radiation free, compact instrumentation, real-time monitoring, easy operation, etc. The maximum excitation/emission values of ICG are 785/821 nm. When ICG is injected into a human body, it rapidly bound to plasma protein, mainly high-density lipoprotein, and generates red-shifted fluorescence (845 nm). ICG in aqueous solution is unstable over time, thus the fresh solution should be used for effective trials. BioActs provides ICG dye for biological research and medical diagnostics and also offers various reactive and functionalized ICG dyes for labeling of antibodies, peptides, proteins and ligands. | |||||||
ICG Dyes |
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| ICG | ICG (water-soluble) |
ICG NHS ester |
ICG Vinylsulfone |
ICG Sulfo-NHS ester |
ICG Isothiocyanate |
ICG Carboxylic acid |
ICG Maleimide |
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| ICG Amine | ICG Thiol |
ICG Dichlorotriazine |
ICG Hydrazide |
ICG Alkyne |
ICG PEG4-Alkyne |
ICG ADIBO |
ICG Azide |
| Quick link (Cat.#) | Series | Quick link (Cat.#) | Series |
| RFP0815 | ICG | RFP0915 | ICG (water-soluble) |
| POS1604 | ICG NHS ester | POA1616 | ICG Vinylsulfone |
| POSN1604 | ICG Sulfo-NHS ester | POI1616 | ICG Isothiocyanate |
| POC1616 | ICG Carboxylic acid | PWM1301 | ICG Maleimid |
| POE1616 | ICG Amine | PWT1301 | ICG Thiol |
| POR2616 | ICG Dichlorotriazine | POH1616 | ICG Hydrazide |
| POK1616 | ICG Alkyne | POG1616 | ICG PEG4-Alkyne |
| DOC1061 | ICG ADIBO | POZ1616 | ICG Azide |
Other Labeling Dyes |
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| BioActs provides other traditionally used dyes such as 5(6)-Carboxyfluorescein (FAM) and 5(6)-Carboxytetramethylrhodamine (TAMRA) dyes for labeling of biomolecules. FAM is one of popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. In addition to relatively high absorptivity, good fluorescence quantum yield and good water solubility, FAM has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. TAMRA fluorophore has been a widely used for preparing bioconjugates, especially fluorescent antibody and avidin derivatives. TAMRA dye is also widely utilized for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore. | |||||
FAM Dye |
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| FAM NHS ester |
FAM Sulfo-NHS ester |
FAM Vinylsulfone |
FAM Maleimide |
FAM Azide |
FAM Hydrazide |
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| FAM PEG4-Alkyne |
FAM ADIBO |
FAM Amine |
FAM Thiol |
FAM Alkyne |
FAM Dichlorotriazine |
| Quick link (Cat.#) | Series | Quick link (Cat.#) | Series |
| CWS1025 | FAM NHS ester | CWSN1025 | FAM Sulfo-NHS ester |
| CWA1020 | FAM Vinylsulfone | CWM1003 | FAM Maleimide |
| CWZ1003 | FAM Azide | CWH1003 | FAM Hydrazide |
| CWK1003 | FAM Alkyne | PWK1701 | FAM PEG4-Alkyne |
| DWF1001 | FAM ADIBO | CWE1003 | FAM Amine |
| CWT1003 | FAM Thiol | CWR2003 | FAM Dichlorotriazine |
TAMRA Dye |
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| TAMRA NHS ester |
TAMRA Sulfo-NHS ester |
TAMRA Vinylsulfone |
TAMRA Maleimide |
TAMRA Azide |
TAMRA Hydrazide |
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| TAMRA Alkyne |
TAMRA PEG4-Alkyne |
TAMRA Adibo |
TAMRA Amine |
TAMRA Thiol |
TAMRA Dichlorotriazine |
| Quick link (Cat.#) | Series | Quick link (Cat.#) | Series |
| KWS1025 | TAMRA NHS ester | KWSN1025 | TAMRA Sulfo-NHS ester |
| KWA1020 | TAMRA Vinylsulfone | KWM1057 | TAMRA Maleimide |
| KWZ1025 | TAMRA Azide | KWH1025 | TAMRA Hydrazide |
| KWK1025 | TAMRA Alkyne | KWG1025 | TAMRA PEG4-Alkyne |
| DWR1001 | TAMRA ADIBO | KWE1025 | TAMRA Amine |
| KWT1057 | TAMRA Thiol | KWR2025 | TAMRA Dichlorotriazine |
qFlamma® Quenchers |
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| Dark quenchers are substances that absorb excitation energy from a fluorophore and dissipate the energy as heat without any native fluorescence. The combination of a fluorescent dye and quencher may act as an on/off switch of fluorescence that the original fluorescence might be restored as the distance between them increases or when their connection is cleaved. In conjunction with fluorophores, dark quenchers are regarded as key components in designing FRET probes, in protease activity assays, nucleic acid hybridization, PCR, etc. The main advantage of dark quencher is the non-emissive excitation and elimination of background fluorescence. BioActs developed a new generation of azo-anthraquinone derivative dark quenchers, qFlamma® quenchers, to overcome issues associated with existing dark quenchers such as stability, quenching ability and water solubility. The absorbance peaks of qFlamma® quenchers completely cover spectral range from visible to near-infrared (NIR) that all of commercially available fluorescent dyes can be effectively quenched. The quenchers display high absorption coefficient together with excellent quenching ability, superb stability and aqueous solubility so that it can easily manipulated in aqueous buffers. The four of qFlamma® quenchers (Black 01, Red, Blue and Orange) series cover the electromagnetic spectrum from 400 nm to NIR region and provides excellent spectral overlap across the entire range of known fluorescent dyes. The complete spectral overlap of qFlamma® quencher series along with their variety of reacting groups enable to design single-tube multiplexing assay system owing their capability for efficient quenching and the preventing cross-talking between dyes. BioActs offers qFlamma® quenchers as universal dark quenchers for the construction of a variety of hybridization and custom-synthesized probes incorporated spectrally paired fluorophores and quenchers for multiplexed bio-analysis, PCR, nucleic acid hybridization, and high-throughput imaging assays. |
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qFlamma® Black01 quencher |
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| NHS ester | Sulfo-NHS ester | Vinylsulfone | Carboxylic acid | Maleimide | Amine | Thiol |
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| Dichlorotriazine | PEG4-Alkyne | ADIBO | Biotin | Alkyne | Azide | Hydrazide |
| Quick link (Cat.#) | Series | Quick link (Cat.#) | Series |
| QWS1001 | qFlamma® Black01 NHS ester | QWSN1001 | qFlamma® Black01 Sulfo-NHS ester |
| QWA1001 | qFlamma® Black01 Vinylsulfone | QWC1001 | qFlamma® Black01 Carboxylic acid |
| QWM1001 | qFlamma® Black01 Maleimide | QWE1001 | qFlamma® Black01 Amine |
| QWT1001 | qFlamma® Black01 Thiol | QWR1001 | qFlamma® Black01 Dichlorotriazine |
| QWG1001 | qFlamma® Black01 PEG4-Alkyne | QWD1001 | qFlamma® Black01 ADIBO |
| QWB1001 | qFlamma® Black01 Biotin | QWK1001 | qFlamma® Black01 Alkyne |
| QWZ1001 | qFlamma® Black01 Azide | QWH1001 | qFlamma® Black01 Hydrazide |
Fluorescent Dyes for Oligonucleotide Synthesis |
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| Fluorescent oligonucleotide probes are developed for the detection and the quantification of target DNA or RNA sequence with the high sensitivity and specificity along with low toxicity. They have been used in a wide range of applications including, FISH, in situ hybridization, sequencing and genotyping. Fluorescent dye and quencher paired molecular beacon probes are widely used in the field of real–time nucleic acid detection and PCR quantification, SNP detection, and clinical diagnostic assays. There are two major strategies for chemical incorporation of fluorescent dyes into an oligonucleotides: direct fluorescence labeling during chemical synthesis and post synthetic fluorescence labeling. The direct oligonucleotide labeling during solid phase synthesis can be achieved via the standard phosphoramidite chemistry containing various spacer arm lengths. Direct labeling during solid phase chemical synthesis might afford high yield but also increases the risk of damaging fluorescence tag from acidic deprotection step. Thus certain fluorescent dyes are not compatible with solid phase chemical synthesis, thus their labeling must be conducted post–synthetically through various labeling methodologies. Post synthetic labeling of a fluorescent dye to an oligonucleotide can be achieved by labeling various activated dyes to modified oligomers having functional groups such as primary amine, thiol, aldehyde, azide, alkyne, or carboxylic acids. Depending on binding target, the fluorophore might be selectively introduced at either the 5′ or 3′ ends of the oligonucleotide as well as randomly incorporated throughout the sequence. BioActs offers fluorescent dye phosphoramidites and fluorescent nucleotide phosphoramidites for direct fluorescence labeling and Flamma NA series dyes for post synthetic fluorescence labeling method. |
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Flamma® NA NHS ester |
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| 553/570 | 590/608 | 583/603 | 646/665 | 686/709 | HEX NA NHS ester | TET NA NHS ESTE |
| Quick link (Cat.#) | Series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PNS1122 | Flamma® 552NA NHS ester | 553 | 570 | Alexa 555, DyLight 549, Cy 3, ATTO 550 |
| PNS1415 | Flamma® 581NA NHS ester | 590 | 608 | Alexa 594, DyLight 594, Cy 3.5 |
| KNS1001 | Flamma® 594NA NHS ester | 583 | 603 | Alexa594, DyLight594, Texas Red-X |
| PNS1215 | Flamma® 648NA NHS ester | 646 | 665 | Alexa647, DyLight650, Cy5 |
| PNS1515 | Flamma® 675NA NHS ester | 689 | 709 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF 680 |
| COS1030 | HEX NA NHS ester | |||
| COS1022 | TET NA NHS ESTER |
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