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ICG NHS ester

Product#: POS1604

$1,007.84

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ICG NHS ester

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Description

ICG NHS ester is an advanced, amine-reactive form of the near-infrared (NIR) fluorescent dye Indocyanine Green (ICG), specifically designed for stable and efficient biomolecule labeling in bioimaging applications. This modified version of ICG combines the exceptional optical properties of the parent compound with enhanced reactivity, making it an invaluable tool for researchers in various fields of life sciences and medical imaging.

The dye can be effectively excited using 750-800 nm laser lines or LED sources, positioning it in the optimal NIR region for deep tissue imaging. This spectral profile allows for visualization of structures several millimeters below the skin surface, significantly reducing background autofluorescence and enhancing signal-to-noise ratios in biological samples.

The NHS ester reactive group readily forms stable amide bonds with primary amines found in proteins, antibodies, and amino-modified oligonucleotides. This reactivity ensures efficient and durable labeling of various biomolecules, including the ε-amino groups of lysine residues and the amine termini of nucleotides.

ICG NHS ester is particularly well-suited for antibody and protein labeling, peptide conjugation, ligand modification, cellular labeling, NIR imaging in vivo and in vitro, and amplification substrate optimization. The versatility of ICG NHS ester makes it an excellent choice for researchers developing new biomarkers, studying protein interactions, or enhancing imaging techniques in fields such as oncology, immunology, and neuroscience.

ICG NHS ester represents a significant advancement in NIR fluorescent labeling technology. Its combination of excellent optical properties, enhanced reactivity, and broad applicability makes it an essential tool for researchers pushing the boundaries of bioimaging and molecular biology. As imaging technologies continue to evolve, ICG NHS ester is poised to play a crucial role in developing new diagnostic and therapeutic approaches in both research and clinical settings.

What are the advantages?
1. Enhanced Reactivity: The NHS ester modification allows for more controlled and efficient conjugation compared to unmodified ICG.
2. Stable Conjugates: Forms chemically stable amide bonds with target molecules, ensuring long-lasting fluorescence signals for extended experimental timeframes.
3. Deep Tissue Imaging: NIR fluorescence enables visualization of structures deep within tissues, ideal for in vivo imaging applications.
4. Reduced Autofluorescence: The NIR spectral range minimizes interference from biological autofluorescence, enhancing signal clarity.
5. Versatility: Suitable for a wide range of biomolecules and applications, from cellular labeling to in vivo imaging.
6. Compatibility: Can be used with various imaging systems designed for NIR fluorescence detection.

Specifications

Fluorophore: ICG
Reactive group: NHS ester
Excitation/Emission Max.(nm): 785/812
Extinction coefficient: ≥ 218,000 cm-1M-1
CF280: 0.05
Appearance: Green Solid
Molecular Weight: 828.03 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

ICG Dyes

Quick link (Cat.#)	Series	Quick link (Cat.#)	Series
RFP0815	ICG	RFP0915	ICG (water-soluble)
POS1604	ICG NHS ester	POA1616	ICG Vinylsulfone
POSN1604	ICG Sulfo-NHS ester	POI1616	ICG Isothiocyanate
POC1616	ICG Carboxylic acid	PWM1301	ICG Maleimid
POE1616	ICG Amine	PWT1301	ICG Thiol
POR2616	ICG Dichlorotriazine	POH1616	ICG Hydrazide
POK1616	ICG Alkyne	POG1616	ICG PEG4-Alkyne
DOC1061	ICG ADIBO	POZ1616	ICG Azide

Background

ICG Dyes

ICG is clinically approved NIR dye and used in medical diagnostics, in vitro, vivo and animal model study. NIR fluorescence allows to observe the deep image from the surface of skin and its usage spreads to a wide range of research fields. ICG fluorescence method is safe and cost effective technique and used in a wide range of medical fields such as breast cancer sentinel lymph node navigation, detecting cerebral vessels, coronary arteries and biliary trees, tracking tumor location, detecting small HCC, etc. The method has several advantages such as radiation free, compact instrumentation, real-time monitoring, easy operation, etc. The maximum excitation/emission values of ICG are 785/821 nm. When ICG is injected into a human body, it rapidly bound to plasma protein, mainly high-density lipoprotein, and generates red-shifted fluorescence (845 nm). ICG in aqueous solution is unstable over time, thus the fresh solution should be used for effective trials. BioActs provides ICG dye for biological research and medical diagnostics and also offers various reactive and functionalized ICG dyes for labeling of antibodies, peptides, proteins and ligands.

Table 1. ICG dye applications

Figure 1. Structure of Indocyanine Green (ICG) dye

Citation & Reference

1. Masashi Gotoh. Development of a canine model of pulmonary emphysema and imaging of the emphysematous lung with infrared thoracoscopy. J Thorac Cardiovasc Surg 126.6 (2003): 1916-21.

2. Aaron M. Mohs. An integrated widefield imaging and spectroscopy system for contrast-enhanced, image-guided resection of tumors. IEEE Trans Biomed Eng 62.5 (2015): 1416-24.

3. Mohammed Hassan. Near Infrared Fluorescence Imaging with ICG in TECAB Surgery Using the da Vinci Si Surgical System in a Canine Model. J Card Surg 27.2 (2012): 158-162.

4. R. C. Benson. Fluorescence properties of indocyanine green as related to angiography. Phys Med Biol 23.1 (1978): 159-63.

5. Mitsuharu Miwa. The Principle of ICG Fluorescence Method. The Open Surgical Oncology Journal 2 (2010): 26-28.

6. Rahman, M. S., Hadrick, K., Chung, S. J., Carley, I., Yoo, J. Y., Nahar, S., ... & Jeong, J. W. (2025). Nanoceria as a non-steroidal anti-inflammatory drug for endometriosis theranostics. Journal of Control Release, 378, 1015–1029.