ICG Hydrazide
Size of product (mg)
-
Trial
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1 mg
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5 mg
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25 mg
ICG Hydrazide
Cat. No. List below
Description
ICG Hydrazide is a carbonyl group-reactive near infrared (NIR) fluorescent dye and used to generate a stable fluorescence signal in bioimaging. NIR fluorescence allows to observe the deep image from the surface of skin and being utilized in a wide range of research fields. The maxima of Ex/Em values are at 785/821 nm. ICG might be excited using 750-800 nm laser line or LED and displays excellent optical property. Hydrazides can label aldehyde and ketone through reductive amination reaction to form an imine linkage. The main labeling target for hydrazides are free reducing sugars on biomolecules, and prior to conjugation, primary and secondary alcohols on polysaccharide and glycoprotein are usually oxidized to aldehyde and ketone. We offer ICG hydrazide for labeling of polysaccharide, glycoprotein and other biomolecules bearing aldehyde or ketone.
- Fluorophore: ICG
- Reactive group: Hydrazide
- Excitation/Emission Max.(nm): 785/812
- Extinction coefficient: ≥ 100,000 cm-1M-1
- CF280: 0.05
- Appearance: Green Solid
- Molecular Weight: 745 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | Series | Quick link (Cat.#) | Series |
| RFP0815 | ICG | RFP0915 | ICG (water-soluble) |
| POS1604 | ICG NHS ester | POA1616 | ICG Vinylsulfone |
| POSN1604 | ICG Sulfo-NHS ester | POI1616 | ICG Isothiocyanate |
| POC1616 | ICG Carboxylic acid | PWM1301 | ICG Maleimid |
| POE1616 | ICG Amine | PWT1301 | ICG Thiol |
| POR2616 | ICG Dichlorotriazine | POH1616 | ICG Hydrazide |
| POK1616 | ICG Alkyne | POG1616 | ICG PEG4-Alkyne |
| DOC1061 | ICG ADIBO | POZ1616 | ICG Azide |
Background
ICG Dyes
ICG is clinically approved NIR dye and used in medical diagnostics, in vitro, vivo and animal model study. NIR fluorescence allows to observe the deep image from the surface of skin and its usage spreads to a wide range of research fields. ICG fluorescence method is safe and cost effective technique and used in a wide range of medical fields such as breast cancer sentinel lymph node navigation, detecting cerebral vessels, coronary arteries and biliary trees, tracking tumor location, detecting small HCC, etc. The method has several advantages such as radiation free, compact instrumentation, real-time monitoring, easy operation, etc. The maximum excitation/emission values of ICG are 785/821 nm. When ICG is injected into a human body, it rapidly bound to plasma protein, mainly high-density lipoprotein, and generates red-shifted fluorescence (845 nm). ICG in aqueous solution is unstable over time, thus the fresh solution should be used for effective trials. BioActs provides ICG dye for biological research and medical diagnostics and also offers various reactive and functionalized ICG dyes for labeling of antibodies, peptides, proteins and ligands.
Table 1. ICG dye applications
Figure 1. Structure of Indocyanine Green (ICG) dye
Citation & Reference
1. Masashi Gotoh. Development of a canine model of pulmonary emphysema and imaging of the emphysematous lung with infrared thoracoscopy. J Thorac Cardiovasc Surg 126.6 (2003): 1916-21.
2. Aaron M. Mohs. An integrated widefield imaging and spectroscopy system for contrast-enhanced, image-guided resection of tumors. IEEE Trans Biomed Eng 62.5 (2015): 1416-24.
3. Mohammed Hassan. Near Infrared Fluorescence Imaging with ICG in TECAB Surgery Using the da Vinci Si Surgical System in a Canine Model. J Card Surg 27.2 (2012): 158-162.
4. R. C. Benson. Fluorescence properties of indocyanine green as related to angiography. Phys Med Biol 23.1 (1978): 159-63.
5. Mitsuharu Miwa. The Principle of ICG Fluorescence Method. The Open Surgical Oncology Journal 2 (2010): 26-28.
