Flamma® 552 Carboxylic acid
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 552 Carboxylic acid
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 550/565 nm
2. High extinction coefficient: ≥ 150,000 cm?¹M?¹
3. Low CF280: 0.07
4. Carboxylic acid functional group
5. Red solid appearance
6. Molecular weight: 672.85 g/mol
7. Soluble in DMF and DMSO
Applications:
1. Bioimaging and fluorescence microscopy
2. Reference standard for dye-conjugates
3. Coupling with primary amines on small molecules or biomolecules
4. Potential use in flow cytometry and fluorescence-based assays
5. Cellular labeling and detection
Advantages:
1. Spectral similarity to popular dyes (Alexa 555, DyLight 549, ATTO 550, Cy3)
2. Excellent optical properties when excited with 532, 543, 546, or 555 nm laser lines
3. Versatile coupling options through standard amide bond formation
4. Potential for conversion to reactive amine form
5. Stable fluorescence signal for extended imaging sessions
- Fluorophore: Flamma® Fluors 552
- Functional group: Carboxylic acid
- Excitation/Emission Max.(nm): 550/565
- Spectrally similar dyes: Alexa555, DyLight549, Cy3, ATTO550
- Extinction coefficient: ≥ 150,000 cm-1M-1
- CF280: 0.07
- Appearance: Red Solid
- Molecular Weight: 672.85 g/mol
- Solubility: DMF, DMSO
- Storage conditions: 4 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWC1101 | Flamma® 552 Carboxylic acid | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWC1201 | Flamma® 648 Carboxylic acid | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWC1501 | Flamma® 675 Carboxylic acid | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWC1308 | Flamma® 749 Carboxylic acid | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWC1603 | Flamma® 774 Carboxylic acid | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Carboxylic acid
Flamma® Fluors Carboxylic acids are non-reactive form of Flamma® Fluors that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid can be converted to a reactive amine form by using standard chemical techniques or coupled to hydrazines. Flamma® Fluors carboxylic acids can be coupled with amines at small molecules or peptides by standard amide bond coupling conditions.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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