Flamma® 774 Carboxylic acid
Size of product (mg)
-
1 mg
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5 mg
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25 mg
Flamma® 774 Carboxylic acid
Cat. No. List below
Description
Flamma® Fluors 774 Carboxylic acid exhibits excellent NIR fluorescence properties, with excitation and emission maxima at 774 nm and 800 nm, respectively. This spectral range allows for deep tissue penetration and minimal autofluorescence background. The dye can be excited using 750 or 785 nm laser lines or dye-pumped laser excitation sources.
This versatile dye is ideal for various applications, including protein and antibody labeling, small molecule conjugation, biomolecule tagging, in vitro and in vivo imaging, and reference standard for dye-conjugates.
The carboxylic acid functional group provides flexibility for further modifications such as direct coupling with primary amines on small molecules or biomolecules using standard amide bond formation techniques and conversion to reactive amine form through standard chemical methods.
Flamma® Fluors 774 Carboxylic acid has been utilized in numerous research applications, including drug delivery systems, cancer theranostics, and arthritis diagnosis. Its high extinction coefficient and favorable spectral properties make it an excellent choice for researchers seeking a reliable NIR fluorescent dye for their studies.
What are the advantages?
1. High signal-to-noise ratio for sensitive detection
2. Stability in various solvents
3. Versatility in labeling strategies
4. Compatibility with common NIR imaging systems
- Fluorophore: Flamma® Fluors 774
- Functional group: Carboxylic acid
- Excitation/Emission Max.(nm): 774/800
- Spectrally similar dyes: Cy7.5, DyLight800, IRDye800
- Extinction coefficient: ≥ 182,000 cm-1M-1
- CF280: 0.087
- Appearance: Green Solid
- Molecular Weight: 929.06 g/mol
- Solubility: DMF, DMSO
- Storage conditions: 4 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWC1101 | Flamma® 552 Carboxylic acid | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWC1201 | Flamma® 648 Carboxylic acid | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWC1501 | Flamma® 675 Carboxylic acid | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWC1308 | Flamma® 749 Carboxylic acid | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWC1603 | Flamma® 774 Carboxylic acid | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Carboxylic acid
Flamma® Fluors Carboxylic acids are non-reactive form of Flamma® Fluors that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid can be converted to a reactive amine form by using standard chemical techniques or coupled to hydrazines. Flamma® Fluors carboxylic acids can be coupled with amines at small molecules or peptides by standard amide bond coupling conditions.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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