Flamma® 749 Carboxylic acid
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 749 Carboxylic acid
Cat. No. List below
Description
Its functional group is carboxylic acid, which can be coupled with primary amines on small molecules or biomolecules using standard amide bond coupling conditions. It is convertible to a reactive amine form using standard chemical techniques.
Flamma® Fluors 749 Carboxylic acid is particularly useful for reference standard for dye-conjugates, custom synthesis of fluorescent probes, and bioimaging studies requiring NIR fluorescence.
To utilize Flamma® Fluors 749 Carboxylic acid effectively, in a coupling reactions, activate the carboxylic acid group using standard coupling reagents. Then react with primary amines under appropriate conditions to form amide bonds and finally for conversion to other reactive forms, employ suitable chemical modification techniques.
The NIR emission of Flamma 749 makes it particularly valuable for in vivo and deep tissue imaging applications. Its spectral properties allow for multiplexing with other fluorophores in multi-color imaging experiments.
The carboxylic acid functionality provides a versatile starting point for creating custom fluorescent probes tailored to specific research needs. This flexibility, combined with the dye's excellent spectral properties, makes Flamma® Fluors 749 Carboxylic acid an invaluable tool for researchers in fields such as molecular biology, biochemistry, and biomedical imaging.
What are the advantages?
1. High brightness: With an extinction coefficient of ≥ 200,000 cm?¹M?¹, Flamma 749 offers exceptional brightness for sensitive detection
2. NIR emission: Ideal for deep tissue imaging with minimal autofluorescence
3. Versatility: Can be converted to various reactive forms for different labeling strategies
4. Stability: Provides a stable fluorescence signal in bioimaging applications
- Fluorophore: Flamma® Fluors 749
- Functional group: Carboxylic acid
- Excitation/Emission Max.(nm): 749/774
- Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750
- Extinction coefficient: ≥ 200,000 cm-1M-1
- CF280: 0.03
- Appearance: Green Solid
- Molecular Weight: 710.90 g/mol
- Solubility: DMF, DMSO
- Storage conditions: 4 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWC1101 | Flamma® 552 Carboxylic acid | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWC1201 | Flamma® 648 Carboxylic acid | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWC1501 | Flamma® 675 Carboxylic acid | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWC1308 | Flamma® 749 Carboxylic acid | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWC1603 | Flamma® 774 Carboxylic acid | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Carboxylic acid
Flamma® Fluors Carboxylic acids are non-reactive form of Flamma® Fluors that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid can be converted to a reactive amine form by using standard chemical techniques or coupled to hydrazines. Flamma® Fluors carboxylic acids can be coupled with amines at small molecules or peptides by standard amide bond coupling conditions.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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