Flamma® 675 Carboxylic acid
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 675 Carboxylic acid
Cat. No. List below
Description
Flamma® Fluors 675 Carboxylic Acid is an advanced near-infrared (NIR) fluorescent dye designed for exceptional versatility in bioimaging applications. This inactive form of the dye, derived from benzindocyanine structure, offers researchers a flexible platform for custom labeling strategies and serves as a valuable reference standard for dye-conjugates.
Key Features:
- Versatile Functionality: Carboxylic acid group allows for custom conjugation strategies
- NIR Spectral Properties: Ex/Em maxima at 675/691 nm, ideal for deep tissue imaging
- High Extinction Coefficient: ≥ 220,000 cm?¹M?¹ for strong fluorescence signals
- Octanoic Acid Attachment: Enhances solubility and reduces aggregation
- Spectral Similarity: Comparable to popular dyes like Alexa 680 and Cy5.5
- Reference Standard: Ideal for quality control of dye-conjugates
Flamma® Fluors 675 Carboxylic Acid offers multiple conjugation approaches:
Direct Amide Coupling: React with primary amines on small molecules or biomolecules using standard coupling reagents (e.g., EDC/NHS).
Amine Conversion: Transform the carboxylic acid into a reactive amine for alternative labeling strategies.
Custom Linker Attachment: Incorporate specialized linkers for specific applications or improved solubility.
While primarily used as a precursor or reference standard, Flamma® Fluors 675 Carboxylic Acid supports various biological studies such as protein labeling by developing custom protein conjugation protocols, small molecule tagging, which creates fluorescent probes for binding studies,
nanoparticle functionalization, which attaches to nanoparticles for advanced imaging applications, and bioconjugate chemistry, which explores novel conjugation strategies for improved in vivo performance.
Flamma® Fluors 675 Carboxylic Acid represents a versatile tool in the arsenal of NIR fluorescent dyes for bioimaging. Its unique combination of a modifiable carboxylic acid group, superior spectral properties, and high extinction coefficient makes it an excellent choice for researchers seeking flexibility in their fluorescence-based experiments. Whether you're developing new conjugation methods, requiring a reliable reference standard, or exploring complex biological systems, this dye offers the adaptability and performance needed to advance your research in the near-infrared spectrum. Its potential for custom modifications and high sensitivity make it an invaluable asset for pushing the boundaries of fluorescence imaging and bioconjugate chemistry.
- Fluorophore: Flamma® Fluors 675
- Functional group: Carboxylic acid
- Excitation/Emission Max.(nm): 675/691
- Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
- Extinction coefficient: ≥ 220,000 cm-1M-1
- CF280: 0.088
- Appearance: Blue Solid
- Molecular Weight: 945.11 g/mol
- Solubility: DMF, DMSO
- Storage conditions: 4 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWC1101 | Flamma® 552 Carboxylic acid | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWC1201 | Flamma® 648 Carboxylic acid | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWC1501 | Flamma® 675 Carboxylic acid | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWC1308 | Flamma® 749 Carboxylic acid | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWC1603 | Flamma® 774 Carboxylic acid | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
- Flexible Conjugation: The carboxylic acid group enables coupling with primary amines using standard amide bond formation techniques.
- Convertible Functionality: Can be transformed into a reactive amine form using standard chemical methods, expanding its versatility.
- Deep Tissue Imaging: NIR emission allows for imaging of deeper tissues with reduced autofluorescence and improved signal-to-noise ratio.
- High Sensitivity: Exceptional extinction coefficient ensures strong fluorescence signals, even at low concentrations.
- Spectral Compatibility: Similar spectral properties to widely used dyes, facilitating integration into existing imaging setups.
Flamma® Fluors Carboxylic acid
Flamma® Fluors Carboxylic acids are non-reactive form of Flamma® Fluors that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid can be converted to a reactive amine form by using standard chemical techniques or coupled to hydrazines. Flamma® Fluors carboxylic acids can be coupled with amines at small molecules or peptides by standard amide bond coupling conditions.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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