Flamma® 749 Maleimide
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 749 Maleimide
Cat. No. List below
Description
Its reactive group is maleimide, which selectively labels thiols of cysteine residues via 1,4-addition pathway. It forms stable thioether linkages with target molecules. It does not typically react with methionine, histidine, or tyrosine. it may react with primary amines under strongly basic conditions.
Flamma 749 Maleimide is particularly useful for antibody labeling (primary and secondary), protein and peptide conjugation, in vitro imaging, in vivo NIR imaging, and fluorescence-based detection methods.
To utilize Flamma 749 Maleimide effectively, ensure target proteins or peptides have accessible cysteine residues. Perform labeling reactions in slightly acidic to neutral pH (6.5-7.5) to maintain selectivity for thiols. Avoid strongly basic conditions to prevent unwanted reactions with primary amines and purify the conjugate to remove unreacted dye. Use the labeled molecule for imaging or other fluorescence-based applications.
The NIR emission of Flamma 749 makes it particularly valuable for in vivo and deep tissue imaging applications. Its spectral properties allow for multiplexing with other fluorophores in multi-color imaging experiments.
The high extinction coefficient and low CF280 value (0.03) indicate that Flamma 749 can provide strong fluorescence signals with minimal interference in protein quantification assays based on absorption at 280 nm.
The maleimide reactive group offers high specificity for thiol groups, making it an excellent choice for site-specific labeling of proteins and peptides. This specificity can be particularly useful in applications where the position of the fluorescent label is critical, such as in studies of protein structure or function.
Flamma® Fluors 749 Maleimide represents a powerful tool in modern biomedical research, offering high sensitivity, specificity, and versatility for a wide range of imaging and labeling applications, particularly where site-specific NIR fluorescence labeling is desired for deep tissue or in vivo imaging.
What are the advantages?
1. Selective Reactivity: Specifically targets cysteine residues, allowing for site-specific labeling
2. High Brightness: With an extinction coefficient of ≥ 200,000 cm?¹M?¹, Flamma 749 offers exceptional brightness for sensitive detection
3. NIR Emission: Ideal for deep tissue imaging with minimal autofluorescence
4. Stability: Forms chemically stable thioether bonds with target molecules
5. Versatility: Suitable for a wide range of biomolecules containing accessible cysteine residues
- Fluorophore: Flamma® Fluors 749
- Reactive group: Maleimide
- Excitation/Emission Max.(nm): 749/774
- Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750
- Extinction coefficient: ≥ 200,000 cm-1M-1
- CF280: 0.03
- Appearance: Green Solid
- Molecular Weight: 833.02 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWM1001 | Flamma® 496 Maleimide | 496 | 520 | Alexa488, FAM |
| CWM1058 | Flamma® 552 Maleimide | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550, CF555 |
| KWM1415 | Flamma® 581 Maleimide | 581 | 596 | Alexa594, DyLight594 |
| KWM1042 | Flamma® 648 Maleimide | 648 | 663 | Alexa647, DyLight650, Cy5, ATTO 647N, CF647 |
| PWM1415 | Flamma® 675 Maleimide | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWM1215 | Flamma® 749 Maleimide | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWM1515 | Flamma® 774 Maleimide | 774 | 806 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors maleimide
Maleimide is an excellent reactive group that can specially label thiol of cysteine residue without interacting with amino functionality. In labeling process, thiol is added to the double bond of maleimide via 1,4-addition pathway to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they are reacted with amines in higher pH than reaction of maleimides. BioActs offers Flamma® Fluors maleimide series as thiol-reactive fluorescence dyes.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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