Flamma® 552 Maleimide
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 552 Maleimide
Cat. No. List below
Description
Key Features
1. High sensitivity for detecting low-abundance biomolecules
2. Excellent optical properties and photostability
3. Selective labeling of thiol groups on cysteine residues
4. Forms stable thioether linkages
5. Compatible with various biomolecules and cellular structures
Applications
1. Labeling of thiols on antibodies, peptides, proteins, and ligands
2. Cellular labeling and detection
3. Fluorescence microscopy
4. Flow cytometry
5. Multicolor imaging (compatible with other fluorophores)
6. Amplification substrate labeling
Advantages
1. High fluorescence quantum yield for bright signals
2. Minimal interaction with other amino acids (methionine, histidine, tyrosine)
3. Versatile for use in various biological studies
4. Maintains good fluorescence activity and stability after conjugation
5. Allows detection of low-abundance biological structures
6. Compatible with most fluorescent equipment
Flamma® Fluors 552 Maleimide offers researchers a powerful tool for precise and sensitive labeling in a wide range of biological applications. Its excellent spectral properties, high photostability, and specific reactivity make it an ideal choice for advanced fluorescence-based studies and imaging techniques.
- Fluorophore: Flamma® Fluors 552
- Reactive group: Maleimide
- Excitation/Emission Max.(nm): 550/565
- Spectrally similar dyes: Alexa555, DyLight549, Cy3, ATTO550, CF555
- Extinction coefficient: ≥ 136,000 cm-1M-1
- CF280: 0.07
- Appearance: Red Solid
- Molecular Weight: 793.3 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWM1001 | Flamma® 496 Maleimide | 496 | 520 | Alexa488, FAM |
| CWM1058 | Flamma® 552 Maleimide | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550, CF555 |
| KWM1415 | Flamma® 581 Maleimide | 581 | 596 | Alexa594, DyLight594 |
| KWM1042 | Flamma® 648 Maleimide | 648 | 663 | Alexa647, DyLight650, Cy5, ATTO 647N, CF647 |
| PWM1415 | Flamma® 675 Maleimide | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWM1215 | Flamma® 749 Maleimide | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWM1515 | Flamma® 774 Maleimide | 774 | 806 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors maleimide
Maleimide is an excellent reactive group that can specially label thiol of cysteine residue without interacting with amino functionality. In labeling process, thiol is added to the double bond of maleimide via 1,4-addition pathway to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they are reacted with amines in higher pH than reaction of maleimides. BioActs offers Flamma® Fluors maleimide series as thiol-reactive fluorescence dyes.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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