Flamma® 648 Maleimide
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 648 Maleimide
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 648/663 nm
2. High extinction coefficient: ≥ 227,000 cm?¹M?¹
3. Excellent photostability and brightness
4. Maleimide reactive group for specific thiol labeling
5. Blue solid appearance
6. Molecular weight: 806.99 g/mol
7. Soluble in DMF and DMSO
Applications:
1. Super-resolution microscopy
2. Single-molecule detection
3. Fluorescence in situ hybridization (FISH)
4. Flow cytometry
5. Confocal laser scanning microscopy
6. Live-cell imaging
7. Multiphoton microscopy
8. Labeling of antibodies, peptides, proteins, ligands, nucleic acids, and cellular organelles
Advantages:
1. Optimal far-red spectrum operation, minimizing autofluorescence
2. Excellent signal-to-noise ratios
3. Visualization of scarce biomolecules with unprecedented clarity
4. Spectral similarity to popular fluorophores (Alexa 647, DyLight 650, Cy5, ATTO 647N, CF647)
5. Seamless integration into existing imaging protocols
6. Highly specific and efficient labeling of thiol groups in biomolecules
7. Minimal off-target labeling for cleaner, more accurate imaging results
8. Formation of exceptionally stable thioether linkages
9. Long-lasting fluorescence signals ideal for extended imaging sessions
10. Excellent performance in complex biological environments
Flamma® Fluors 648 Maleimide offers unparalleled performance in the far-red spectrum, with excitation and emission maxima at 648 nm and 663 nm, respectively. This spectral range minimizes autofluorescence and provides excellent signal-to-noise ratios, crucial for high-quality imaging results. The dye's remarkable extinction coefficient of ≥ 227,000 cm?¹M?¹ ensures brilliant fluorescence intensity, even at low concentrations, enabling the visualization of scarce biomolecules with unprecedented clarity.
The maleimide reactive group enables highly specific and efficient labeling of thiol groups in biomolecules, particularly cysteine residues, via the 1,4-addition pathway. This selectivity ensures minimal off-target labeling, resulting in cleaner, more accurate imaging results. The formed thioether linkages are exceptionally stable, providing long-lasting fluorescence signals ideal for extended imaging sessions.
Engineered to perform in complex biological environments, Flamma® Fluors 648 Maleimide exhibits excellent solubility in DMF and DMSO, coupled with stability in aqueous solutions. This versatility makes it perfect for labeling a diverse range of biomolecules, including antibodies, peptides, proteins, ligands, nucleic acids, and cellular organelles.
Researchers can seamlessly integrate this dye into existing imaging setups, thanks to its spectral similarity to widely-used fluorophores like Alexa 647, DyLight 650, Cy5, ATTO 647N, and CF647. This compatibility allows for easy adoption in various advanced bioimaging techniques, from super-resolution microscopy to live-cell imaging.
For optimal results, store Flamma® Fluors 648 Maleimide at -20°C and protect it from light. With its superior brightness, excellent photostability, and high specificity, this dye empowers researchers to achieve unprecedented clarity and precision in their cellular and molecular imaging studies.
- Fluorophore: Flamma® Fluors 648
- Reactive group: Maleimide
- Excitation/Emission Max.(nm): 648/663
- Spectrally similar dyes: Alexa647, DyLight650, Cy5, ATTO 647N, CF647
- Extinction coefficient: ≥ 227,000 cm-1M-1
- CF280: 0.03
- Appearance: Blue Solid
- Molecular Weight: 806.99 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWM1001 | Flamma® 496 Maleimide | 496 | 520 | Alexa488, FAM |
| CWM1058 | Flamma® 552 Maleimide | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550, CF555 |
| KWM1415 | Flamma® 581 Maleimide | 581 | 596 | Alexa594, DyLight594 |
| KWM1042 | Flamma® 648 Maleimide | 648 | 663 | Alexa647, DyLight650, Cy5, ATTO 647N, CF647 |
| PWM1415 | Flamma® 675 Maleimide | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWM1215 | Flamma® 749 Maleimide | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWM1515 | Flamma® 774 Maleimide | 774 | 806 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
- Superior Brightness: With its high extinction coefficient, Flamma® Fluors 648 Maleimide offers exceptional brightness for clear, vivid imaging results.
- Excellent Photostability: Enjoy prolonged imaging sessions with minimal photobleaching, ensuring consistent and reliable data collection.
- Versatile Compatibility: Seamlessly integrate this dye into your existing imaging setups, thanks to its spectral similarity to widely-used fluorophores.
- High Specificity: Achieve precise labeling of your target molecules with minimal background interference, enhancing the accuracy of your results.
- Optimized for Cellular Studies: Specially formulated for excellent performance in cellular environments, making it ideal for a wide range of biological research applications.
Flamma® Fluors maleimide
Maleimide is an excellent reactive group that can specially label thiol of cysteine residue without interacting with amino functionality. In labeling process, thiol is added to the double bond of maleimide via 1,4-addition pathway to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they are reacted with amines in higher pH than reaction of maleimides. BioActs offers Flamma® Fluors maleimide series as thiol-reactive fluorescence dyes.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
1. Xu, Peisheng. Zwitterionic chitosan derivatives for pH-sensitive stealth coating. Biomacromolecules 11.9 (2010): 2352-2358.
2. Ibrahim, Basma M. A strategy to deliver genes to cystic fibrosis lungs: a battle with environment. Journal of controlled release 155.2 (2011): 289-295.
3. Oh, Keun Sang. Accurate sequential detection of primary tumor and metastatic lymphatics using a temperature-induced phase transition nanoparticulate system. International journal of nanomedicine 9 (2014): 2955.
4. Yhee, Ji Young. Tumor-targeting transferrin nanoparticles for systemic polymerized siRNA delivery in tumor-bearing mice. Bioconjugate chemistry 24.11 (2013): 1850-1860.
5. Yoon, Hong Yeol. Glycol chitosan nanoparticles as specialized cancer therapeutic vehicles: Sequential delivery of doxorubicin and Bcl-2 siRNA. Scientific reports 4 (2014).
6. Ryu, Ju Hee. Early diagnosis of arthritis in mice with collagen?induced arthritis, using a fluorogenic matrix metalloproteinase 3–specific polymeric probe. Arthritis & Rheumatism 63.12 (2011): 3824-3832.
7. Hollis, Christin P. In vivo investigation of hybrid paclitaxel nanocrystals with dual fluorescent probes for cancer theranostics. Pharmaceutical research 31.6 (2014): 1450-1459.
8. Koo, Heebeom. The movement of self-assembled amphiphilic polymeric nanoparticles in the vitreous and retina after intravitreal injection. Biomaterials 33.12 (2012): 3485-3493.
9. Zhu, Lei. Real-time monitoring of caspase cascade activation in living cells. Journal of controlled release 163.1 (2012): 55-62.
10. Yoon, Hong Yeol. Bioreducible hyaluronic acid conjugates as siRNA carrier for tumor targeting. Journal of Controlled Release 172.3 (2013): 653-661.
11. Yhee, Ji Young. Cancer-targeted MDR-1 siRNA delivery using self-cross-linked glycol chitosan nanoparticles to overcome drug resistance. Journal of Controlled Release 198 (2015): 1-9.
12. Park, Jin Woo. Wide-Ranged Fluorescent Molecular Weight Size Markers for Electrophoresis. Bulletin of the Korean Chemical Society 34.1 (2013): 29-30.
13. Huang, Xinglu. Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad?Spectrum Nanoquencher. Angewandte Chemie International Edition 51.7 (2012): 1625-1630.
