Flamma® 496 Maleimide
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 496 Maleimide
Cat. No. List below
Description
Key Features
1. Bright green fluorescence with excitation/emission maxima at 496/520 nm
2. Thiol-reactive maleimide group for specific labeling of cysteine residues
3. High sensitivity for detection of low-abundance biomolecules
4. Excellent optical properties, compatible with 488 nm laser excitation
5. Strong absorption, high fluorescence quantum yield, and high photostability
6. Maintains good fluorescence activity and stability after conjugation
Applications
1. Labeling of thiols on antibodies, peptides, proteins, and ligands
2. Cellular labeling and detection
3. Fluorescence microscopy
4. Flow cytometry
5. Cell-based assays
6. Amplification substrate labeling
Advantages
1. Selective labeling of cysteine residues via 1,4-addition pathway
2. Forms stable thioether linkages
3. Minimal cross-reactivity with other amino acids (methionine, histidine, tyrosine)
4. Compatible with most fluorescent equipment
5. Covers a broad spectral range from UV to NIR when used with other Flamma® Fluors
6. Allows detection of low-abundance biological structures with great sensitivity
7. Offers improved solubility and enhanced signal-to-noise ratios in various applications
Flamma® Fluors 496 Maleimide is part of the broader Flamma® Fluors family, which offers a range of dyes covering the full spectral range from UV to NIR. These dyes are equipped with various reactive groups, including NHS esters, vinylsulfones, and click chemistry-compatible functionalities, making them versatile tools for diverse biological research applications.
- Fluorophore: Flamma® Fluors 496
- Reactive group: Maleimide
- Excitation/Emission Max.(nm): 496/520
- Spectrally similar dyes: Alexa 488, FAM
- Extinction coefficient: ≥ 64,000 cm-1M-1
- CF280: 0.12
- Appearance: Yellow Solid
- Molecular Weight: 536.44 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWM1001 | Flamma® 496 Maleimide | 496 | 520 | Alexa488, FAM |
| CWM1058 | Flamma® 552 Maleimide | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550, CF555 |
| KWM1415 | Flamma® 581 Maleimide | 581 | 596 | Alexa594, DyLight594 |
| KWM1042 | Flamma® 648 Maleimide | 648 | 663 | Alexa647, DyLight650, Cy5, ATTO 647N, CF647 |
| PWM1415 | Flamma® 675 Maleimide | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWM1215 | Flamma® 749 Maleimide | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWM1515 | Flamma® 774 Maleimide | 774 | 806 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors maleimide
Maleimide is an excellent reactive group that can specially label thiol of cysteine residue without interacting with amino functionality. In labeling process, thiol is added to the double bond of maleimide via 1,4-addition pathway to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they are reacted with amines in higher pH than reaction of maleimides. BioActs offers Flamma® Fluors maleimide series as thiol-reactive fluorescence dyes.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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