Flamma® 774 Maleimide
Size of product (mg)
-
1 mg
-
5 mg
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25 mg
Flamma® 774 Maleimide
Cat. No. List below
Description
The maleimide group selectively reacts with thiols of cysteine residues via a 1,4-addition pathway, forming a stable thioether linkage. This specificity allows for targeted labeling without interfering with other amino acid residues such as methionine, histidine, or tyrosine. However, under strongly basic conditions, maleimides may react with primary amines.
Flamma® Fluors 774 Maleimide is particularly suited for protein labeling, antibody conjugation, nucleic acid labeling, in vitro imaging, in vivo NIR imaging, fluorescence-based detection methods, and ligand labeling.
Flamma® Fluors 774 Maleimide has been utilized in numerous research applications, including drug delivery systems, cancer theranostics, and arthritis diagnosis. Its high extinction coefficient (≥ 182,000 cm?¹M?¹) and favorable spectral properties make it an excellent choice for researchers seeking a reliable NIR fluorescent dye for their studies.
When using Flamma® Fluors 774 Maleimide, researchers should consider the pH of the labeling reaction, as strongly basic conditions may lead to undesired reactions with primary amines. Additionally, the dye's performance in different solvents and its stability under various experimental conditions should be evaluated for optimal results in specific applications.
What are the advantages:
1. High specificity for thiol groups
2. Stable fluorescence signal
3. NIR emission for deep tissue penetration
4. Minimal autofluorescence background
5. Versatility in labeling various biomolecules
- Fluorophore: Flamma® Fluors 774
- Reactive group: Maleimide
- Excitation/Emission Max.(nm): 774/806
- Spectrally similar dyes: Cy7.5, DyLight800, IRDye800
- Extinction coefficient: ≥ 182,000 cm-1M-1
- CF280: 0.1
- Appearance: Green Solid
- Molecular Weight: 1051.19 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWM1001 | Flamma® 496 Maleimide | 496 | 520 | Alexa488, FAM |
| CWM1058 | Flamma® 552 Maleimide | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550, CF555 |
| KWM1415 | Flamma® 581 Maleimide | 581 | 596 | Alexa594, DyLight594 |
| KWM1042 | Flamma® 648 Maleimide | 648 | 663 | Alexa647, DyLight650, Cy5, ATTO 647N, CF647 |
| PWM1415 | Flamma® 675 Maleimide | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWM1215 | Flamma® 749 Maleimide | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWM1515 | Flamma® 774 Maleimide | 774 | 806 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors maleimide
Maleimide is an excellent reactive group that can specially label thiol of cysteine residue without interacting with amino functionality. In labeling process, thiol is added to the double bond of maleimide via 1,4-addition pathway to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they are reacted with amines in higher pH than reaction of maleimides. BioActs offers Flamma® Fluors maleimide series as thiol-reactive fluorescence dyes.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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