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Flamma® 675 Isothiocyanate

Product#: KWI1515

$8,118.72

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Flamma® 675 Isothiocyanate

Cat. No. List below

Description

Flamma® Fluors 675 Isothiocyanate is a cutting-edge near-infrared (NIR) fluorescent dye designed for superior performance in bioimaging applications. This reactive dye, derived from benzindocyanine structure, offers exceptional stability and versatility, making it an ideal choice for researchers seeking high-quality fluorescence signals in biological studies.

Key Features:

Stable Reactivity: Moderately reactive isothiocyanate group for controlled labeling
Solvent Compatibility: Stable in water and most organic solvents
Spectral Properties: Ex/Em maxima at 675/691 nm, comparable to popular dyes like Alexa 680 and Cy5.5
NIR Emission: Ideal for deep tissue imaging with minimal background interference
Versatile Applications: Suitable for labeling antibodies, peptides, proteins, ligands, and amplification substrates
Optimized for Cellular Studies: Excellent for cellular labeling and detection

Flamma® Fluors 675 Isothiocyanate excels in various bioimaging applications, including cellular labeling (optimized for high-resolution cellular imaging and detection), protein conjugation (efficiently tags antibodies and other proteins), peptide labeling (ideal for fluorescent tagging of peptides), ligand binding studies (excellent for fluorescence-based binding assays and amplification substrate labeling (enhances sensitivity in amplification-based detection methods).

For optimal conjugation results with Flamma® Fluors 675 Isothiocyanate, please adhere to the following:

pH Requirement: Use a buffer with pH above 9 for efficient conjugation
Comparison to NHS Esters: While NHS ester labeling typically occurs at pH 8.3, isothiocyanates require a higher pH for optimal reactivity
Buffer Selection: Carbonate or borate buffers are recommended for maintaining the required alkaline pH

Flamma® Fluors 675 Isothiocyanate is particularly well-suited for live Cell Imaging, which is excellent for labeling cell surface proteins or internalized biomolecules, immunofluorescence, which is good for high-quality labeling of antibodies for sensitive detection in tissue sections, protein Trafficking Studies, which track labeled proteins through cellular compartments with minimal background, and multiplexing experiments, which combine with other fluorophores for multi-color imaging studies.

Flamma® Fluors 675 Isothiocyanate represents a significant advancement in NIR fluorescent dyes for bioimaging. Its unique combination of controlled reactivity, stability, and superior spectral properties makes it an excellent choice for researchers demanding high-quality, reliable results in their fluorescence-based experiments. Whether you're conducting cellular labeling, protein conjugation, or exploring complex biological systems, this dye offers the performance and versatility needed to push the boundaries of your research in the near-infrared spectrum. Its optimized properties for cellular studies make it an invaluable tool for researchers working at the forefront of biological imaging and detection.

Specifications

Fluorophore: Flamma® Fluors 675
Reactive group: Isothiocyanate
Excitation/Emission Max.(nm): 675/691
Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT
Extinction coefficient: ≥ 130,000 cm-1M-1
CF280: 0.11
Appearance: Blue Solid
Molecular Weight: 1029.25 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
PWI1001	Flamma® 496 Isothiocyanate	494	520	Alexa488, FITC, Cy2
PWI1122	Flamma® 552 Isothiocyanate	550	564	Alexa555, DyLight549, Cy3, ATTO550
PWI1415	Flamma® 581 Isothiocyanate	578	593	Alexa594, DyLight594
KWI1215	Flamma® 648 Isothiocyanate	648	663	Alexa647, DyLight650, Cy5
KWI1515	Flamma® 675 Isothiocyanate	675	691	Alexa680, DyLight680, Cy5.5, IRDye680LT
PWI1308	Flamma® 749 Isothiocyanate	749	774	Alexa750, DyLight755, Cy7.5, IRDye750
PWI1603	Flamma® 774 Isothiocyanate	774	800	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

What are the advantages?

Controlled Reactivity: The isothiocyanate group offers moderate reactivity, allowing for precise control during the labeling process.
Stable Conjugation: Forms reasonably stable thiourea linkages with primary amines, ensuring long-lasting fluorescent signals.
Broad Solvent Compatibility: Maintains stability in water and most organic solvents, providing flexibility in experimental design.
Spectral Similarity: Comparable spectral properties to widely used dyes like Alexa 680, Cy5.5, IRDye 680LT, and DyLight 680, enabling easy integration into existing imaging protocols.
Deep Tissue Imaging: NIR emission allows for imaging of deeper tissues with reduced autofluorescence and improved signal-to-noise ratio.

Flamma® Fluors Isothiocyanate

BioActs offers a series of Flamma® Fluors isothiocyanates, which are moderately reactive but quite stable in water and most organic solvents. Isothiocyanates form reasonably stable thiourea linkage upon reaction with amines. Whereas labeling of protein with NHS esters can typically be done at pH 8.3, conjugation for isothiocyanates usually require pH above 9. This basic isothiocyanate labeling condition may be a factor for working with basic-environment tolerable biomolecules that DNA and most polysaccharides can be modified in a relatively basic pH.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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