Flamma® 552 Alkyne
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 552 Alkyne
Cat. No. List below
Description
Flamma® Fluors 552 Alkyne is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent of bright yellow dye induced from cyanine structure and used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 550/564 nm, similar to that of Alexa 555, DyLight 549, ATTO 550 and Cy3. Flamma 552 might be excited using 532, 543, 546 or 555 nm laser lines and displays excellent optical property. Flamma 552 alkyne couples with an azide to form 1,4-disubstituted 1,2,3-triazole inside of living systems without interfering native biochemical processes. Prior to perform CuAAC, the azide functionality should be introduced onto counterpart biomolecule by means of chemical or genetic modification. We offer Flamma Fluors 552 alkyne as a click chemistry reagent dye for cellular imaging and nucleotide functionalization.
- Fluorophore: Flamma® Fluors 552
- Reactive group: Alkyne
- Excitation/Emission Max.(nm): 550/564
- Spectrally similar dyes: Alexa555, DyLight549, Cy3, ATTO550
- Extinction coefficient: ≥ 130,000 cm-1M-1
- CF280: 0.069
- Appearance: Red Solid
- Molecular Weight: 709.92 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWK1001 | Flamma® 496 Alkyne | 496 | 520 | Alexa488, FITC, Cy2 |
| PWK1122 | Flamma® 552 Alkyne | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWK1415 | Flamma® 581 Alkyne | 581 | 596 | Alexa594, DyLight594 |
| PWK1215 | Flamma® 648 Alkyne | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWK1515 | Flamma® 675 Alkyne | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWK1301 | Flamma® 749 Alkyne | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWK1603 | Flamma® 774 Alkyne | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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