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Flamma® 648 Alkyne

Product#: PWK1215

$1,336.00

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5 mg
25 mg

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Flamma® 648 Alkyne

Cat. No. List below

Description

Flamma® Fluors 648 Alkyne is a state-of-the-art far-red fluorescent dye specifically designed for copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reactions in bioimaging applications. This innovative reagent combines the power of click chemistry with exceptional fluorescence properties, offering researchers a versatile tool for cellular imaging and biomolecule labeling.

Key Features and Benefits:

Optimal Spectral Properties: Excitation/emission maxima at 648/663 nm, comparable to popular dyes like Alexa 647, Cy5, and DyLight 650.
Versatile Excitation: Compatible with 593 or 633 nm laser lines, providing flexibility in imaging setups.
Superior Brightness: High extinction coefficient of ≥ 227,000 cm-1M-1 ensures vivid fluorescence signals.
Minimal Protein Interference: Low CF280 value of 0.024 reduces interference with protein absorbance measurements.
Bioorthogonal Labeling: Forms 1,4-disubstituted 1,2,3-triazoles without disrupting native biochemical processes.

Flamma® Fluors 648 Alkyne excels in various applications, including cellular imaging (ideal for live-cell and fixed-cell microscopy), nucleotide functionalization (enables specific labeling of modified nucleic acids), protein labeling (excellent for tracking proteins in complex biological systems), and biomolecule tracking (suitable for studying dynamics of various biomolecules in living systems).

Its chemistry advantage is the alkyne reactive group allows for highly specific and efficient labeling through CuAAC reactions, which are bioorthogonal (reactions occur without interfering with native biological processes), has high specificity (selective labeling of azide-modified biomolecules) and has mild reaction conditions (compatible with a wide range of biological systems).

For best results, introduce azide functionality to target biomolecules via chemical or genetic modification. Perform CuAAC reaction under optimized conditions (e.g., copper catalyst, reducing agent) and purify labeled biomolecules using standard chromatographic techniques.

Specifications

Fluorophore: Flamma® Fluors 648
Reactive group: Alkyne
Excitation/Emission Max.(nm): 648/663
Spectrally similar dyes: Alexa647, DyLight650, Cy5
Extinction coefficient: ≥ 227,000 cm-1M-1
CF280: 0.024
Appearance: Blue Solid
Molecular Weight: 721.93 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
CWK1001	Flamma® 496 Alkyne	496	520	Alexa488, FITC, Cy2
PWK1122	Flamma® 552 Alkyne	550	564	Alexa555, DyLight549, Cy3, ATTO550
KWK1415	Flamma® 581 Alkyne	581	596	Alexa594, DyLight594
PWK1215	Flamma® 648 Alkyne	648	663	Alexa647, DyLight650, Cy5
PWK1515	Flamma® 675 Alkyne	674	691	Alexa680, DyLight680, Cy5.5, IRDye680LT
PWK1301	Flamma® 749 Alkyne	749	774	Alexa750, DyLight755, Cy7.5, IRDye750
PWK1603	Flamma® 774 Alkyne	774	800	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

Why Choose Flamma® Fluors 648 Alkyne?

Cutting-Edge Technology: Combines click chemistry with far-red fluorescence for advanced bioimaging.
Exceptional Brightness: High extinction coefficient for clear, vivid signals.
Versatility: Suitable for various biomolecules and imaging techniques.
Minimal Background: Low CF280 value ensures high signal-to-noise ratio.

Flamma^® Fluors for Click Chemistry

The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma^® Fluors Alkyne dyes for CuAAC, Flamma^® Fluors ADIBO products for SPAAC and Flamma^® Fluors Azide dyes for both CuAAC and SPAAC.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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