Flamma® 749 Alkyne
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 749 Alkyne
Cat. No. List below
Description
The dye has excitation and emission maxima at 749 nm and 774 nm, respectively, placing it in the NIR region. These spectral properties are comparable to other NIR dyes like Alexa 750, Cy7, IRDye 750, and DyLight 755. Flamma 749 can be excited using a 750 nm laser line or dye-pumped laser, with emission occurring in the NIR region, which is highly permeable to biological tissues. This makes it ideal for imaging applications requiring deep tissue penetration and low autofluorescence.
Flamma® Fluors 749 Alkyne reacts with azides through CuAAC to form a stable 1,4-disubstituted 1,2,3-triazole linkage. This reaction is highly selective and efficient under mild conditions, making it suitable for use in living systems without interfering with native biochemical processes. To perform CuAAC, the counterpart biomolecule must first be functionalized with an azide group via chemical or genetic modification.
Flamma® Fluors 749 Alkyne is particularly useful for bioimaging (generates stable fluorescence signals for cellular and tissue imaging), click chemistry
(enables site-specific labeling of azide-functionalized biomolecules), nucleotide functionalization (facilitates the modification of nucleotides for imaging or tracking purposes, and protein & glycan labeling (used to label proteins or glycans modified with azides).
The labeling process typically involves:
Functionalizing the target biomolecule with an azide group (via chemical or genetic methods).
Performing CuAAC with Flamma® Fluors 749 Alkyne in the presence of a copper (I) catalyst.
Utilizing the labeled biomolecules for imaging or other analytical applications.
Flamma® Fluors 749 Alkyne advantages:
1. High Specificity: The CuAAC reaction is highly selective for azides, minimizing off-target reactions.
2. Biocompatibility: Suitable for use in living systems without disrupting native biochemical pathways.
3. NIR Advantages: Reduced photobleaching, lower background fluorescence, and enhanced tissue penetration compared to visible light fluorophores.
4. Multiplexing Potential: Can be used alongside other fluorophores in different spec
- Fluorophore: Flamma® Fluors 749
- Reactive group: Alkyne
- Excitation/Emission Max.(nm): 749/774
- Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750
- Extinction coefficient: ≥ 170,000 cm-1M-1
- Appearance: Green Solid
- Molecular Weight: 747.96 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWK1001 | Flamma® 496 Alkyne | 496 | 520 | Alexa488, FITC, Cy2 |
| PWK1122 | Flamma® 552 Alkyne | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWK1415 | Flamma® 581 Alkyne | 581 | 596 | Alexa594, DyLight594 |
| PWK1215 | Flamma® 648 Alkyne | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWK1515 | Flamma® 675 Alkyne | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWK1301 | Flamma® 749 Alkyne | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWK1603 | Flamma® 774 Alkyne | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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