Flamma® 581 Amine
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 581 Amine
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 578/593 nm
2. High extinction coefficient: ≥ 109,000 cm?¹M?¹
3. CF280: 0.18
4. Primary amine functional group attached through a spacer
5. Purple solid appearance
6. Molecular weight: 961.15 g/mol
7. Soluble in DMF and DMSO
Applications:
1. Bioimaging and fluorescence microscopy
2. Coupling with carboxylic acids on small molecules or biomolecules
3. Reference standard for dye-conjugates
4. Potential use in flow cytometry and fluorescence-based assays
5. Cellular labeling and detection
7. Nucleotide functionalization
Advantages:
1. Spectral similarity to popular dyes (Alexa 594, DyLight 594)
2. Excellent optical properties when excited with 561, 568, or 578 nm laser lines
3. Versatile coupling options through standard amide bond formation
4. Stable fluorescence signal for extended imaging sessions
5. Potential for use in multi-color imaging experiments
6. High sensitivity for detecting low-abundance biological structures
- Fluorophore: Flamma® Fluors 581
- Functional group: Primary amine
- Excitation/Emission Max.(nm): 578/593
- Spectrally similar dyes: Alexa594, DyLight594
- Extinction coefficient: ≥ 109,000 cm-1M-1
- CF280: 0.18
- Appearance: Purple Solid
- Molecular Weight: 961.15 g/mol
- Solubility: DMF, DMSO
- Storage conditions: 4 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWE1001 | Flamma® 496 Amine | 496 | 520 | Alexa488, FITC, Cy2 |
| PWE1122 | Flamma® 552 Amine | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWE1415 | Flamma® 581 Amine | 578 | 593 | Alexa594, DyLight594 |
| PWE1215 | Flamma® 648 Amine | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWE1515 | Flamma® 675 Amine | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWE1301 | Flamma® 749 Amine | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWE1603 | Flamma® 774 Amine | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Amine
Flamma® Fluors Amine dyes have an attached primary amine, which connected through a spacer, and can be used as a reference standard for dye-conjugates. These amine dyes can be conjugated with carboxylic acids at small molecules or peptides by standard amide bond coupling conditions.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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