Flamma® 675 Amine
Product#: PWE1515
$1,100.80
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Availability:
Ships in 1-2 Weeks
Flamma® 675 Amine
Cat. No. List below
Description
Flamma® Fluors 675 Amine is a cutting-edge near-infrared (NIR) fluorescent dye engineered for advanced bioimaging applications. This versatile fluorophore, derived from a benzindocyanine structure, offers exceptional stability and optical properties for generating reliable fluorescence signals.
Key features:
1. Excitation/Emission maxima: 675/691 nm
2. High extinction coefficient: ≥ 200,000 cm?¹M?¹
3. Low CF280: 0.091
4. Primary amine functional group attached through a spacer
5. Blue solid appearance
6. Molecular weight: 987.19 g/mol
7. Soluble in DMF and DMSO
Applications:
1. Bioimaging and fluorescence microscopy
2. In vitro and in vivo imaging studies
3. Coupling with carboxylic acids on small molecules or biomolecules
4. Reference standard for dye-conjugates
5. Protein labeling
6. Nucleic acid tagging
7. Antibody conjugation
8. Peptide modification
9. Nanoparticle functionalization
10. Deep-tissue and whole-body imaging
11. Potential use in fluorescence-guided surgery
Advantages:
1. Spectral similarity to popular dyes (Alexa 680, DyLight 680, Cy5.5, IRDye 680LT, CF680)
2. Excellent optical properties when excited with a 633 nm laser line
3. Versatile coupling options through standard amide bond formation
4. Stable fluorescence signal for extended imaging sessions
5. High sensitivity for detecting low-abundance biological structures
6. Minimal interference with protein absorbance measurements
7. Enhanced tissue penetration and reduced autofluorescence
8. Compatibility with the "near-infrared optical window" (650-900 nm) for improved in vivo imaging
9. Reduced phototoxicity and light scattering in biological samples
Key features:
1. Excitation/Emission maxima: 675/691 nm
2. High extinction coefficient: ≥ 200,000 cm?¹M?¹
3. Low CF280: 0.091
4. Primary amine functional group attached through a spacer
5. Blue solid appearance
6. Molecular weight: 987.19 g/mol
7. Soluble in DMF and DMSO
Applications:
1. Bioimaging and fluorescence microscopy
2. In vitro and in vivo imaging studies
3. Coupling with carboxylic acids on small molecules or biomolecules
4. Reference standard for dye-conjugates
5. Protein labeling
6. Nucleic acid tagging
7. Antibody conjugation
8. Peptide modification
9. Nanoparticle functionalization
10. Deep-tissue and whole-body imaging
11. Potential use in fluorescence-guided surgery
Advantages:
1. Spectral similarity to popular dyes (Alexa 680, DyLight 680, Cy5.5, IRDye 680LT, CF680)
2. Excellent optical properties when excited with a 633 nm laser line
3. Versatile coupling options through standard amide bond formation
4. Stable fluorescence signal for extended imaging sessions
5. High sensitivity for detecting low-abundance biological structures
6. Minimal interference with protein absorbance measurements
7. Enhanced tissue penetration and reduced autofluorescence
8. Compatibility with the "near-infrared optical window" (650-900 nm) for improved in vivo imaging
9. Reduced phototoxicity and light scattering in biological samples
Specifications
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Fluorophore: Flamma® Fluors 675
- Functional group: Primary amine
- Excitation/Emission Max.(nm): 675/691
- Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
- Extinction coefficient: ≥ 200,000 cm-1M-1
- CF280: 0.091
- Appearance: Blue Solid
- Molecular Weight: 987.19 g/mol
- Solubility: DMF, DMSO
- Storage conditions: 4 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWE1001 | Flamma® 496 Amine | 496 | 520 | Alexa488, FITC, Cy2 |
| PWE1122 | Flamma® 552 Amine | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWE1415 | Flamma® 581 Amine | 578 | 593 | Alexa594, DyLight594 |
| PWE1215 | Flamma® 648 Amine | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWE1515 | Flamma® 675 Amine | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWE1301 | Flamma® 749 Amine | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWE1603 | Flamma® 774 Amine | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
What are the advantages?
- Stability: Provides consistent and reliable fluorescence signals in bioimaging applications.
- Versatility: Compatible with various conjugation methods and biomolecules.
- NIR Emission: Allows for deep tissue penetration in biological samples.
- Spectral Compatibility: Similar spectral properties to widely used NIR dyes, facilitating integration into existing protocols.
Flamma® Fluors Amine
Flamma® Fluors Amine dyes have an attached primary amine, which connected through a spacer, and can be used as a reference standard for dye-conjugates. These amine dyes can be conjugated with carboxylic acids at small molecules or peptides by standard amide bond coupling conditions.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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