Flamma® 774 Amine
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 774 Amine
Cat. No. List below
Description
Flamma® Fluors 774 Amine is particularly suited for protein labeling, small molecule conjugation, biomolecule tagging, in vitro & in vivo imaging, development of custom fluorescent probes, and fluorescence-based assays and detection methods.
The primary amine functional group provides flexibility for further modifications such as direct coupling with carboxylic acids on small molecules or biomolecules using standard amide bond formation techniques and potential for reaction with other electrophilic groups, expanding its labeling capabilities.
Flamma® Fluors 774 Amine represents a powerful tool for researchers in life sciences, offering superior NIR fluorescence properties and flexible conjugation options for advanced imaging and detection applications. Its high extinction coefficient, favorable spectral properties, and versatility make it an excellent choice for a wide range of biological research and diagnostic applications, including challenging in vivo NIR imaging studies and the development of custom fluorescent probes.
What are the advantages?
1. High Signal-to-Noise Ratio: Provides clear and distinct signals, crucial for sensitive detection applications.
2. NIR Fluorescence: Emits in the NIR region, allowing for deep tissue imaging with minimal autofluorescence background.
3. Versatility: Compatible with various excitation sources, including 750 nm or 785 nm laser lines and dye-pumped lasers.
4. Flexible Conjugation: The primary amine group allows for coupling with carboxylic acids on small molecules or biomolecules.
5. Reference Standard: Can be utilized as a reference standard for dye-conjugates.
- Fluorophore: Flamma® Fluors 774
- Functional group: Primary amine
- Excitation/Emission Max.(nm): 774/800
- Spectrally similar dyes: Cy7.5, DyLight800, IRDye800
- Extinction coefficient: ≥ 200,000 cm-1M-1
- CF280: 0.093
- Appearance: Green Solid
- Molecular Weight: 971.15 g/mol
- Solubility: DMF, DMSO
- Storage conditions: 4 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWE1001 | Flamma® 496 Amine | 496 | 520 | Alexa488, FITC, Cy2 |
| PWE1122 | Flamma® 552 Amine | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWE1415 | Flamma® 581 Amine | 578 | 593 | Alexa594, DyLight594 |
| PWE1215 | Flamma® 648 Amine | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWE1515 | Flamma® 675 Amine | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWE1301 | Flamma® 749 Amine | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWE1603 | Flamma® 774 Amine | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Amine
Flamma® Fluors Amine dyes have an attached primary amine, which connected through a spacer, and can be used as a reference standard for dye-conjugates. These amine dyes can be conjugated with carboxylic acids at small molecules or peptides by standard amide bond coupling conditions.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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