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Flamma® 648 Amine

Product#: PWE1215

$1,100.80

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1 mg
5 mg
25 mg

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Flamma® 648 Amine

Cat. No. List below

Description

Flamma® Fluors 648 Amine is a cutting-edge far-red fluorescent dye designed for advanced bioimaging applications. This versatile fluorophore, derived from a cyanine structure, offers exceptional stability and optical properties for generating reliable fluorescence signals.

Key features:
1. Excitation/Emission maxima: 648/663 nm
2. High extinction coefficient: ≥ 250,000 cm?¹M?¹
3. Low CF280: 0.024
4. Primary amine functional group attached through a spacer
5. Blue solid appearance
6. Molecular weight: 726.95 g/mol
7. Soluble in DMF and DMSO

Applications:

1. Bioimaging and fluorescence microscopy
2. Coupling with carboxylic acids on small molecules or biomolecules
3. Protein labeling
4. Nucleic acid tagging
5. Antibody conjugation
6. Peptide modification
7. Potential use in fluorescence-guided surgery
8. Deep-tissue and whole-body imaging

Advantages:

1. Spectral similarity to popular dyes (Alexa647, DyLight650, Cy5)
2. Excellent optical properties when excited with 593 or 633 nm laser lines
3. Versatile coupling options through standard amide bond formation
4. Stable fluorescence signal for extended imaging sessions
5. Minimal interference with protein absorbance measurements
6. High sensitivity for detecting low-abundance biological structures
7. Enhanced tissue penetration and reduced autofluorescence
8. Compatibility with the "near-infrared optical window" (650-950 nm) for improved in vivo imaging

Specifications

Fluorophore: Flamma® Fluors 648
Functional group: Primary amine
Excitation/Emission Max.(nm): 648/663
Spectrally similar dyes: Alexa647, DyLight650, Cy5
Extinction coefficient: ≥ 250,000 cm-1M-1
CF280: 0.024
Appearance: Blue Solid
Molecular Weight: 726.95 g/mol
Solubility: DMF, DMSO
Storage conditions: 4 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
CWE1001	Flamma® 496 Amine	496	520	Alexa488, FITC, Cy2
PWE1122	Flamma® 552 Amine	550	565	Alexa555, DyLight549, Cy3, ATTO550
KWE1415	Flamma® 581 Amine	578	593	Alexa594, DyLight594
PWE1215	Flamma® 648 Amine	648	663	Alexa647, DyLight650, Cy5
PWE1515	Flamma® 675 Amine	675	691	Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
PWE1301	Flamma® 749 Amine	749	774	Alexa750, DyLight755, Cy7.5, IRDye750
PWE1603	Flamma® 774 Amine	774	800	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

Key Features:
Exceptional Stability: Generate reliable, long-lasting fluorescence signals for extended imaging experiments.
Versatile Coupling: The primary amine group, attached via a spacer, allows easy conjugation with carboxylic acids on small molecules and biomolecules.
Optimal Spectral Properties: With excitation/emission maxima at 648/663 nm, Flamma® Fluors 648 Amine perfectly aligns with popular far-red imaging techniques.
High Compatibility: Spectrally similar to Alexa 647, Cy5, and DyLight 650, ensuring seamless integration into existing protocols.
Powerful Performance: Boasting an extinction coefficient of ≥ 250,000 cm?¹M?¹, this dye delivers exceptional brightness and sensitivity.

Flamma^® Fluors Amine

Flamma^® Fluors Amine dyes have an attached primary amine, which connected through a spacer, and can be used as a reference standard for dye-conjugates. These amine dyes can be conjugated with carboxylic acids at small molecules or peptides by standard amide bond coupling conditions.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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