Flamma® 675NA NHS ester
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 675NA NHS ester
Cat. No. List below
Description
Key Features:
1. Excitation/Emission Max: 686/709 nm
2. Extinction coefficient: 138,000 cm^-1M^-1
3. Molecular Weight: 680.85 g/mol
4. Molecular Formula: C44H46N3O4
5. Appearance: Blue Solid
6. Solubility: DMF, DMSO
7. Storage: -20°C, protected from light
Applications:
1. Bioimaging of low-abundance biomolecules
2. Oligonucleotide labeling in solid-phase synthesis
3. Protein conjugation
4. In vitro imaging of fixed cells
5. Flow cytometry
6. Fluorescence microscopy
Advantages:
1. NIR emission for deep tissue penetration
2. Compatibility with 633 nm laser excitation
3. High sensitivity for detecting low-abundance biomolecules
4. Stable amide bond formation with target molecules
5. Versatile labeling of proteins, peptides, and oligonucleotides
6. Low background interference due to NIR emission
7. Excellent for multiplexing experiments
Flamma 675NA NHS ester readily reacts with amino groups of proteins (e.g., ε-amino groups of lysine) or amine-modified nucleotides, forming a chemically stable amide bond. This property makes it ideal for labeling biomolecules and incorporating fluorescent tags into oligonucleotide synthesis. The dye's NIR emission in the biologically permeable region allows for enhanced tissue penetration and reduced autofluorescence, making it particularly suitable for in vivo and in vitro imaging applications.
- Fluorophore: Flamma Fluors 675NA
- Reactive group: NHS ester
- Excitation/Emission Max.(nm): 686/709
- Spectrally similar dyes: Alexa 680, DyLight 680, Cy 5.5, IRDye 680LT, CF 680
- Extinction coefficient: 138,000 cm-1M-1
- Appearance: Blue Solid
- Molecular Weight: 680.85 g/mol
- Molecular Formula: C44H46N3O4
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | Series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PNS1122 | Flamma® 552NA NHS ester | 553 | 570 | Alexa 555, DyLight 549, Cy 3, ATTO 550 |
| PNS1415 | Flamma® 581NA NHS ester | 590 | 608 | Alexa 594, DyLight 594, Cy 3.5 |
| KNS1001 | Flamma® 594NA NHS ester | 583 | 603 | Alexa594, DyLight594, Texas Red-X |
| PNS1215 | Flamma® 648NA NHS ester | 646 | 665 | Alexa647, DyLight650, Cy5 |
| PNS1515 | Flamma® 675NA NHS ester | 689 | 709 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF 680 |
| COS1030 | HEX NA NHS ester | |||
| COS1022 | TET NA NHS ESTER |
Background
Fluorescent Dyes for Oligonucleotide Synthesis
Fluorescent oligonucleotide probes are developed for the detection and the quantification of target DNA or RNA sequence with the high sensitivity and specificity along with low toxicity. They have been used in a wide range of applications including, FISH, in situ hybridization, sequencing and genotyping. Fluorescent dye and quencher paired molecular beacon probes are widely used in the field of real–time nucleic acid detection and PCR quantification, SNP detection, and clinical diagnostic assays.
There are two major strategies for chemical incorporation of fluorescent dyes into an oligonucleotides: direct fluorescence labeling during chemical synthesis and post synthetic fluorescence labeling. The direct oligonucleotide labeling during solid phase synthesis can be achieved via the standard phosphoramidite chemistry containing various spacer arm lengths. Direct labeling during solid phase chemical synthesis might afford high yield but also increases the risk of damaging fluorescence tag from acidic deprotection step. Thus certain fluorescent dyes are not compatible with solid phase chemical synthesis, thus their labeling must be conducted post–synthetically through various labeling methodologies.
Post synthetic labeling of a fluorescent dye to an oligonucleotide can be achieved by labeling various activated dyes to modified oligomers having functional groups such as primary amine, thiol, aldehyde, azide, alkyne, or carboxylic acids. Depending on binding target, the fluorophore might be selectively introduced at either the 5′ or 3′ ends of the oligonucleotide as well as randomly incorporated throughout the sequence. BioActs offers fluorescent dye phosphoramidites and fluorescent nucleotide phosphoramidites for direct fluorescence labeling and Flamma NA series dyes for post synthetic fluorescence labeling method.
Citation & Reference
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