Flamma® 774 Dichlorotriazine
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 774 Dichlorotriazine
Cat. No. List below
Description
Flamma® Fluors 774 Dichlorotriazine exhibits a unique reactivity profile:
-Hydroxyl Reactivity: Specifically designed to react with hydroxyl groups.
-Reaction Mechanism: Hydroxyls irreversibly displace one of the chlorines on the triazine ring, forming a stable aryl ether linkage.
-pH Requirement: Optimal reaction occurs at pH > 9 in aqueous solutions.
-Selectivity: One of the few reactive groups capable of directly labeling polysaccharides and alcohols in aqueous solutions.
It can be used in applications such as polysaccharide labeling: (ideal for tagging complex carbohydrates and glycans), alcohol labeling (suitable for modifying alcohols on various biomolecules), cellular imaging (enables high-contrast visualization of labeled structures in cells), and biomolecule detection (provides a stable fluorescence signal with high signal-to-noise ratio).
The dye's ability to react with polysaccharides and alcohols in aqueous solutions sets it apart from many other fluorescent labels. Its NIR properties make it excellent for applications requiring deep tissue penetration or where background fluorescence is a concern. The high signal-to-noise ratio enhances detection sensitivity in complex biological samples.
Flamma® Fluors 774 Dichlorotriazine represents a significant advancement in NIR fluorescent labeling technology, offering researchers a powerful tool for exploring complex biological systems with high specificity and sensitivity.
What are the advantages?
1. High Sensitivity: The high extinction coefficient ensures strong signal generation.
2. Low Protein Interference: The low CF280 value minimizes interference with protein absorbance measurements.
3. Versatility: Compatible with various imaging systems due to its spectral similarity to widely used NIR dyes.
4. Stability: Generates stable fluorescence signals, ideal for long-term imaging studies.
Unique Reactivity: Offers labeling options for molecules traditionally challenging to modify
- Fluorophore: Flamma® Fluors 774
- Reactive group: Dichlorotriazine
- Excitation/Emission Max.(nm): 774/800
- Spectrally similar dyes: Cy7.5, DyLight800, IRDye800, CF770
- Extinction coefficient: ≥ 141,000 cm-1M-1
- CF280: 0.1
- Appearance: Green Solid
- Molecular Weight: 1119 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | Series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWR2112 | Flamma® 552 Dichlorotriazine | 550 | 564 | Alexa555, DyLight550, Cy3, ATTO550 |
| KWR2415 | Flamma® 581 Dichlorotriazine | 578 | 593 | Alexa594, DyLight594 |
| PWR2215 | Flamma® 648 Dichlorotriazine | 648 | 667 | Alexa647, DyLight650, Cy5 |
| PWR2515 | Flamma® 675 Dichlorotriazine | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWR2301 | Flamma® 749 Dichlorotriazine | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWR2603 | Flamma® 774 Dichlorotriazine | 774 | 800 | DyLight800, Cy7.5, IRDye800, CF770 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Dichlorotrazine
Flamma® Fluors dichlorotriazine is for labeling hydroxyl groups. Hydroxyls irreversibly displace one of chlorine at triazine ring via SNAr reaction pathway to yield an aryl ether linkage. Dichlorotriazines are among the few reactive groups that are reported to react directly with polysaccharides and other alcohols in aqueous solution, provided that the pH is >9 and other nucleophiles are not present.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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