Flamma® 675 Dichlorotriazine
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 675 Dichlorotriazine
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 675/691 nm
2. High extinction coefficient: ≥ 144,000 cm?¹M?¹
3. Low CF280: 0.105
4. Dichlorotriazine reactive group for specific hydroxyl labeling
5. Blue solid appearance
6. Molecular weight: 1135.14 g/mol
7. Soluble in water, DMF, and DMSO
Applications:
1. Labeling of polysaccharides and alcohols on biomolecules
2. Cellular labeling and detection
3. In vitro and in vivo imaging studies
4. Glycobiology research
5. Cell surface labeling
6. Studies involving carbohydrate-based interactions
7. Deep-tissue imaging
Advantages:
1. NIR spectral properties allow for deep tissue penetration and minimal autofluorescence
2. Direct reactivity with polysaccharides and alcohols in aqueous solutions (pH >9)
3. Forms stable aryl ether linkages through irreversible chlorine displacement
4. Excellent optical properties when excited using a 633 nm laser line
5. Spectral similarity to popular NIR dyes (Alexa 680, Cy5.5, IRDye 680LT, DyLight 680, CF680)
6. Suitable for multi-color imaging experiments
7. Flexibility in experimental setups due to solubility in both aqueous and organic solvents
- Fluorophore: Flamma® Fluors 675
- Reactive group: Dichlorotriazine Excitation/Emission Max.(nm): 675/691
- Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
- Extinction coefficient: ≥ 144,000 cm-1M-1
- CF280: 0.105
- Appearance: Blue Solid
- Molecular Weight: 1135.14 g/mol
- Solubility: Water, DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | Series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWR2112 | Flamma® 552 Dichlorotriazine | 550 | 564 | Alexa555, DyLight550, Cy3, ATTO550 |
| KWR2415 | Flamma® 581 Dichlorotriazine | 578 | 593 | Alexa594, DyLight594 |
| PWR2215 | Flamma® 648 Dichlorotriazine | 648 | 667 | Alexa647, DyLight650, Cy5 |
| PWR2515 | Flamma® 675 Dichlorotriazine | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWR2301 | Flamma® 749 Dichlorotriazine | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWR2603 | Flamma® 774 Dichlorotriazine | 774 | 800 | DyLight800, Cy7.5, IRDye800, CF770 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
- Unique Reactivity: One of the few reactive groups capable of directly labeling polysaccharides and alcohols in aqueous solutions.
- NIR Emission: Allows for deep tissue penetration and reduced autofluorescence in biological samples.
- Stability: Provides consistent and reliable fluorescence signals.
- Versatility: Compatible with a wide range of biomolecules and imaging techniques.
Flamma® Fluors Dichlorotrazine
Flamma® Fluors dichlorotriazine is for labeling hydroxyl groups. Hydroxyls irreversibly displace one of chlorine at triazine ring via SNAr reaction pathway to yield an aryl ether linkage. Dichlorotriazines are among the few reactive groups that are reported to react directly with polysaccharides and other alcohols in aqueous solution, provided that the pH is >9 and other nucleophiles are not present.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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