0.1M Glycine Quenching Buffer with PBS
Catalog No.: DCP-GQPBS0.1X
Size: 500 mL
Non-sterile: Filtered 0.1-micron membrane once, 0.04-micron membrane once
Descriptions:
0.1M Glycine Quenching Buffer with PBS is a ready-to-use solution designed to rapidly quench unreacted aldehyde groups following formaldehyde or paraformaldehyde fixation. Glycine functions as a quenching agent through its amino group (-NH
2), which reacts with unreacted formaldehyde molecules and prevents further cross-linking reactions with cellular proteins. This mechanism is crucial because it stops the fixation process at a controlled time point, preventing over-fixation that could compromise epitope recognition by antibodies. This buffer helps stop crosslinking at the desired time point, reduces background signal, and preserves epitope integrity for high-quality immunostaining and imaging results.
Features:
- Optimized for aldehyde-based fixatives (formaldehyde, paraformaldehyde).
- Helps reduce background and autofluorescence in IF/ICC and microscopy workflows.
- Compatible with downstream applications including IF, ICC, IHC, flow cytometry, and certain ChIP workflows.
- Prepared in physiological buffer to maintain cell and tissue morphology.
Applications:
- Quenching residual formaldehyde or paraformaldehyde after cell or tissue fixation.
- Reducing fixative-derived background fluorescence in immunofluorescence and confocal microscopy.
- Improving signal-to-noise ratio in immunocytochemistry and immunohistochemistry.
- Stabilizing samples prior to permeabilization, blocking, or antibody incubation steps.
- General use wherever controlled termination of aldehyde crosslinking is required.
Composition:
| Ingredients |
Concentration |
| Glycine |
0.1 M |
| Sodium Chloride |
137 mM |
| Disodium Hydrogen Phosphate |
10 mM |
| Potassium Chloride |
2.7 mM |
| Monootassium Phosphate |
1.8 mM |
Recommended Use:
- Fix cells or tissue with aldehyde-based fixative according to your protocol.
- Remove fixative and rinse once with PBS or appropriate wash buffer.
- Incubate samples with 0.1M Glycine Quenching Buffer for 10–30 minutes at room temperature.
- Proceed to permeabilization, blocking, or antibody staining steps as required.
Quality Control
- Apperance: Clear Solution
- pH: 7.4
- Sterility: Filtered 0.1-micron membrane once, 0.04-micron membrane once in a non-sterile environment
Storage and Shelf Life
- Storage: 4°C (Protect from light and excessive heat)
- Shelf Life: 1 year
* All DiagnoCine Precision Sterile media are filtered-sterilized with a 0.1-micron filtration two times and 0.04 micron once. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
