THUNDERBIRD® SYBR® qPCR Master Mix, Trial (Only 1 Per Customer)
THUNDERBIRD® SYBR® qPCR Master Mix *Only 1 Per Customer*
A highly efficient 2× master mix for real-time PCR with SYBR® Green I — all components included except ROX, probe, and primers — for fast setup, high specificity, and reproducible quantification. An improved version of Realtime PCR Master Mix (Code No. QPK-201).
Cold-chain shipping required. This product has ICE or DRY ICE shipping requirements — do not select Ground Shipping. The $1.00 listing applies to the sample (Trial) size only; please inquire. Only 1 Per Customer.
Tip: THUNDERBIRD is optimized for PCR amplicon size less than 500 bp and routine PCR purposes. Do not use it for PCR to amplify targets larger than 500 base pairs.
Description
THUNDERBIRD® SYBR® qPCR Mix is a highly efficient 2× Master Mix for real-time PCR using SYBR® Green I. The master mix contains all required components, except for ROX reference dye, probe, and primers. 50× ROX reference dye is individually supplied with this kit. The master mix facilitates fast reaction setup and improves the reproducibility of experimental data.
This product is an improved version of Realtime PCR Master Mix (Code No. QPK-201). In particular, reaction specificity and PCR efficiency are enhanced.
Ordering Information
Available pack sizes — all stored at -20 °C.
Features
The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The specificity for the detection of low-copy targets is enhanced.
The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer (patent pending) in the master mix.
High specificity and a robust enzyme enable the detection of a broad dynamic range.
Applicable to most real-time cyclers (i.e. block type and glass capillary type) including ABI, Bio-Rad, Roche, and TaKaRa. The 50× ROX reference dye in the kit can be applied to real-time cyclers that require a passive reference dye.
Specifications
Components
| Code No. | Contents | Amount |
|---|---|---|
| QPS-201T | THUNDERBIRD® SYBR® qPCR Mix | 1 ml × 1 |
| 50× ROX reference dye | 50 µl × 1 | |
| QPS-201 | THUNDERBIRD® SYBR® qPCR Mix | 1.67 ml × 3 |
| 50× ROX reference dye | 250 µl × 1 | |
| QPS-201X5 | THUNDERBIRD® SYBR® qPCR Mix | (1.67 ml × 3) × 5 |
| 50× ROX reference dye | 250 µl × 5 |
Compatible Real-Time Instruments
Application Data
Example 1. Comparison of sensitivity and efficiency of the SYBR® Green I assay
β-actin mRNA was detected with serially diluted cDNA from HeLa cell total RNA. THUNDERBIRD® SYBR® qPCR Mix [Code No. QPS-201] showed greater sensitivity and efficiency than other kits (companies A and B).
Example 2. Comparison of PCR efficiency
Norovirus G1 and human GAPDH genes were detected using serially diluted cDNA samples by SYBR® Green I and TaqMan real-time PCR. THUNDERBIRD® qPCR Master Mix showed greater efficiency than other reagents.
Example 3. Verification of measurement accuracy
Human GAPDH genes were detected using serially 20.5-fold diluted cDNA synthesised from HeLa cell total RNA by SYBR® Green I assay. THUNDERBIRD® qPCR Master Mix successfully detected the differences between dilutions.
Comparison with Company T
The graphs below show the qPCR results of HIV and b2mg genes. The Ct values were compared between SYBR qPCR master mix products from Company T and Toyobo. Toyobo’s THUNDERBIRD SYBR qPCR Mix shows superior and consistent performance with significantly lower Ct values on all dilutions and samples tested.
Comparison with Company R
Data kindly provided by NIH/NHLBI.
Real-Time PCR Kits
DiagnoCine offers TOYOBO® Taq DNA Polymerases in the form of Master Mix (Probe), SYBR Green Master Mix (Plus), THUNDERBIRD qPCR (Probe), and THUNDERBIRD SYBR qPCR formats. All Taq DNA Polymerases provide the highest specificity by Hot Start technology, excellent passive reference, and fit well for glass capillaries for many purposes.
- Real-time PCR Master Mix is a probe assay type with the highest hot start specificity, fast routine PCR experiments, and works well for GC-rich regions. This is for routine short-length PCR targets and not suitable for the long target (>10kb length; KOD is best for long-range size PCR) PCR. It can be used for crude samples, but KOD is best for crude sample amplifications. This product’s strongest feature is the high specificity and Probe master mix for routine end-point PCR experiments.
- SYBR Green Realtime PCR Master Mix (plus for a hot start) is not as specific as the other Taq polymerases (i.e. Probe type or THUNDERBIRD SYBR are more specific) master mix, but this brand provides an excellent passive reference and fits for glass capillary use. It is designed to work well for routine short-range PCR and light GC-rich regions and fairly clean crude samples.
- THUNDERBIRD Probe qPCR Mix is a probe assay type with hot start specificity, good passive reference, and glass capillary type PCR experiments. The strength is the highest specificity with low noise bands for difficult PCR and routine experiments such as genotyping and colony screening. Promised for best specificity and fast PCR speed but not made for a long-range PCR (>10kb length; KOD is best for long amplicons more than 10kb). It can unwind the GC-rich region and crude samples can be used (KOD is the best for crude sample use). This type is best for all routine laboratory PCR with short-mid range amplicons, looking for fast PCR time but with the strongest specificity and not dealing with PCR noise bands.
- THUNDERBIRD SYBR qPCR Mix is an SYBR assay type with a strong specificity but slightly less specific than Probe qPCR. It has passive reference and glass capillary compatibility. The product is designed for a routine PCR with short to mid-range amplicons for a short and fast PCR time. The best performance is expected with partially clean crude samples and a fair level of GC-rich complexity.
