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Flamma® 496 Vinylsulfone

Product#: CWA1001

$797.28

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25 mg

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Flamma® 496 Vinylsulfone

Cat. No. List below

Description

Flamma® Fluors 496 Vinylsulfone is a cutting-edge, pH-insensitive bright green fluorescent dye engineered for stable and sensitive bioimaging applications. Developed by BioActs' leading technology, this reactive dye generates consistent fluorescence signals across a wide pH range and at high temperatures, making it an invaluable tool for researchers in cellular and molecular biology.

With excitation and emission maxima at 496 nm and 520 nm respectively, Flamma 496 is spectrally similar to Alexa 488 and FAM. It can be efficiently excited using a 488 nm laser line, offering excellent compatibility with common fluorescence imaging systems.

Key Features:
1. Bright green fluorescence with Ex/Em maxima at 496/520 nm
2. pH insensitive across a wide range
3. High temperature stability
4. Excellent photostability and optical properties
5. Efficient conjugation to biomolecules via vinylsulfone chemistry
6. High dye-to-protein (d/P) ratio for enhanced signal strength
7. High extinction coefficient (≥ 66,000 cm?¹M?¹) for improved sensitivity

Applications:
1. Fluorescence microscopy
2. Flow cytometry
3. Protein and nucleic acid labeling
4. Labeling of antibodies, peptides, proteins, and ligands
5. Amplification substrate optimization
6. Applications requiring pH stability
7. Cellular labeling and detection

Advantages:
1. High sensitivity for detecting low-abundance biomolecules
2. Stable fluorescence signal in bioimaging
3. Versatile labeling capabilities for various biomolecules
4. Compatible with common 488 nm laser excitation sources
5. Optimized for cellular labeling and detection
6. Forms stable amino linkages with primary amines
7. Low CF280 value (0.14) for minimal interference with protein absorbance

The vinylsulfone reactive group of Flamma 496 readily forms stable amino linkages with primary amines of amino-modified oligonucleotides or proteins. This chemistry ensures efficient and durable labeling of target biomolecules under various experimental conditions.

Flamma® Fluors 496 Vinylsulfone offers researchers a powerful tool for advanced fluorescence labeling, combining high sensitivity, excellent optical properties, and versatile conjugation chemistry. Its unique pH insensitivity and high-temperature stability make it particularly suitable for challenging experimental conditions, providing clear signals with minimal background interference and simplified experimental procedures across a wide range of biological studies.

Specifications

Fluorophore: Flamma® Fluors 496
Reactive group: Vinylsulfone
Excitation/Emission Max.(nm): 496/520
Spectrally similar dyes: Alexa488, FAM
Extinction coefficient: ≥ 66,000 cm-1M-1
CF280: 0.14
Appearance: Yellow Solid
Molecular Weight: 531.48 g/mol
Solubility: DMF, DMSO
Storage conditions: 4 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
CWA1002	Flamma® 488 Vinylsulfone	495	519	Alexa488, Cy2
CWA1001	Flamma® 496 Vinylsulfone	496	520	Alexa488, FAM
PWA1122	Flamma® 552 Vinylsulfone	550	564	Alexa555, DyLight549, Cy3, ATTO550, CF555
PWA1415	Flamma® 581 Vinylsulfone	581	596	Alexa594, DyLight594
KOA1001	Flamma® 594 Vinylsulfone	590	617	Alexa594, DyLight594
PWA1215	Flamma® 648 Vinylsulfone	648	683	Alexa647, DyLight650, Cy5
PWA1515	Flamma® 675 Vinylsulfone	675	691	Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
PWA1308	Flamma® 749 Vinylsulfone	749	774	Alexa750, DyLight755, Cy7, IRDye750
PWA1603	Flamma® 774 Vinylsulfone	774	795	Cy7.5, CF 770, DyLight 800, IRDye800
POA1803	Flamma® 800 Vinylsulfone	774	789	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide
and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

Flamma^® Fluors Vinylsulfone

BioActs’ leading technology developed highly reactive yet stable and pH insensitive Flamma® Fluors Vinylsulfone dyes. Vinylsulfone reactive group mainly react with the primary amine of biomolecules under a wide range of pH from 5 to 10 though the labeling reaction rate is proportional to the basicity of medium. Vinylsulfone dyes are stable in both organic and aqueous solution at higher temperature, thus they can be dissolved in water avoiding organic solvents such as DMSO or DMF, and the aqueous stock solution can be stored in a refrigerator. Vinylsulfone reacting group is added by an amine via 1,2 addition pathway to form a stable amino linkage between dye and the biomolecule, therefore none of side product would be generated during conjugation step. BioActs recommends Flamma® Fluors Vinylsulfone for labeling biomolecules in harsh conditions such as having less reactive amines, running the reaction in prolong period, at high temperature or under strong basic or acidic environment.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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