Flamma® 594 Vinylsulfone
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 594 Vinylsulfone
Cat. No. List below
Description
Key Features:
1. pH-insensitive reactive form
2. Stable across a wide pH range and at high temperatures
3. Excitation/Emission maxima at 590/617 nm
4. Compatible with 578 nm laser line excitation
5. Excellent optical properties
6. High sensitivity for low-abundance biomolecule detection
7. High dye-to-protein (d/P) ratio
Applications:
1. Fluorescence microscopy and confocal imaging
2. Flow cytometry
3. Fluorescence-based assays
4. Labeling of antibodies, peptides, proteins, ligands, and amplification substrates
5. Single-molecule detection
6. Cellular labeling and detection
7. Fluorescence correlation spectroscopy
Advantages:
1. Versatile conjugation: Readily reacts with primary amines of amino-modified oligonucleotides or proteins
2. Stable linkage: Forms a robust amino linkage between the dye and biomolecules
3. Spectral similarity: Comparable to Alexa 594 and DyLight 594, allowing for easy integration into existing protocols
4. Superior brightness: Maintains excellent fluorescence after conjugation
5. Enhanced stability: Improved photostability compared to traditional dyes
6. Reduced background: Lower non-specific binding for improved signal-to-noise ratios
7. Wide pH stability: Functional across a broad range of pH conditions
8. Temperature resistance: Maintains stability at high temperatures
Flamma® Fluors 594 Vinylsulfone is part of the broader Flamma® Fluors series, which covers a wide spectral range from UV to NIR. These dyes are characterized by strong absorption, high fluorescence quantum yield, and excellent photostability. They maintain good fluorescence activity and stability after conjugation to biomolecules, allowing for the detection of low-abundance biological structures with great sensitivity.
The vinylsulfone reactive group of Flamma® 594 offers unique advantages, including stability in a wide range of pH conditions and at high temperatures. This makes it an ideal choice for challenging experimental conditions and ensures consistent performance across various applications in biological studies.
Researchers can leverage Flamma® 594 Vinylsulfone for a variety of fluorescence-based techniques, including in vitro and in vivo imaging, molecular biology studies, and advanced biomedical research. Its compatibility with common fluorescence imaging systems and its superior optical properties make it a valuable tool for cutting-edge life science research and medical diagnostics.
The dye's ability to be conjugated to low-abundance biomolecules with great sensitivity and high d/P ratio enables precise and sensitive detection in various experimental setups. This feature is particularly useful in studies involving rare or low-expression proteins, making Flamma® 594 Vinylsulfone an excellent choice for researchers working on challenging detection problems.
- Fluorophore: Flamma® Fluors 594
- Reactive group: Vinylsulfone
- Excitation/Emission Max.(nm): 590/617
- Spectrally similar dyes: Alexa594, DyLight594
- Extinction coefficient: ≥ 83,000 cm-1M-1
- CF280: 0.56
- Appearance: Purple Solid
- Molecular Weight: 839.95 g/mol
- Solubility: DMF
- Storage conditions: 4 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWA1002 | Flamma® 488 Vinylsulfone | 495 | 519 | Alexa488, Cy2 |
| CWA1001 | Flamma® 496 Vinylsulfone | 496 | 520 | Alexa488, FAM |
| PWA1122 | Flamma® 552 Vinylsulfone | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550, CF555 |
| PWA1415 | Flamma® 581 Vinylsulfone | 581 | 596 | Alexa594, DyLight594 |
| KOA1001 | Flamma® 594 Vinylsulfone | 590 | 617 | Alexa594, DyLight594 |
| PWA1215 | Flamma® 648 Vinylsulfone | 648 | 683 | Alexa647, DyLight650, Cy5 |
| PWA1515 | Flamma® 675 Vinylsulfone | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWA1308 | Flamma® 749 Vinylsulfone | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWA1603 | Flamma® 774 Vinylsulfone | 774 | 795 | Cy7.5, CF 770, DyLight 800, IRDye800 |
| POA1803 | Flamma® 800 Vinylsulfone | 774 | 789 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide
and hydrophobic substances. - High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Vinylsulfone
BioActs’ leading technology developed highly reactive yet stable and pH insensitive Flamma® Fluors Vinylsulfone dyes. Vinylsulfone reactive group mainly react with the primary amine of biomolecules under a wide range of pH from 5 to 10 though the labeling reaction rate is proportional to the basicity of medium. Vinylsulfone dyes are stable in both organic and aqueous solution at higher temperature, thus they can be dissolved in water avoiding organic solvents such as DMSO or DMF, and the aqueous stock solution can be stored in a refrigerator. Vinylsulfone reacting group is added by an amine via 1,2 addition pathway to form a stable amino linkage between dye and the biomolecule, therefore none of side product would be generated during conjugation step. BioActs recommends Flamma® Fluors Vinylsulfone for labeling biomolecules in harsh conditions such as having less reactive amines, running the reaction in prolong period, at high temperature or under strong basic or acidic environment.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
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