Flamma® 749 Azide
Size of product (mg)
-
1 mg
-
5 mg
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25 mg
Flamma® 749 Azide
Cat. No. List below
Description
The azide reactive group is connected to the dye through an amino propyl linkage. Flamma 749 azide couples with an alkyne to form 1,4-disubstituted 1,2,3-triazole, a stable linkage crucial for various biological applicationsIts applications are cellular imaging, nucleotide functionalization. and in vivo bioimaging.
To utilize Flamma® Fluors 749 Azide in click chemistry reactions, do the following:
1. Introduce alkyne functionality to the target biomolecule through chemical or genetic modification.
2. Perform the CuAAC reaction, coupling the azide group of Flamma 749 with the introduced alkyne.
The resulting 1,4-disubstituted 1,2,3-triazole forms a stable link between the fluorescent dye and the biomolecule of interest.
Flamma® Fluors 749 Azide represents a powerful tool in the arsenal of modern bioimaging techniques, offering researchers the ability to visualize and track biomolecules with high specificity and minimal interference in living systems. Its versatility and excellent spectral properties make it an ideal choice for a wide range of biological and biomedical research applications.
What are the advantages?
1. NIR Fluorescence: Emits in the biological tissue permeable NIR region, allowing for deep tissue imaging with minimal autofluorescence background.
2. Biocompatibility: The CuAAC reaction can occur inside living systems without interfering with native biochemical processes.
3. Versatility: Compatible with various excitation sources, including 750 nm laser lines and dye-pumped lasers.
4. High Stability: Generates a stable fluorescence signal, crucial for long-term imaging studies.
- Fluorophore: Flamma® Fluors 749
- Reactive group: Azide
- Excitation/Emission Max.(nm): 749/774
- Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750
- Extinction coefficient: ≥ 170,000 cm-1M-1
- CF280: 0.035
- Appearance: Green Solid
- Molecular Weight: 793.01 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWZ1001 | Flamma® 496 Azide | 496 | 520 | Alexa488, FITC, Cy2 |
| PWZ1122 | Flamma® 552 Azide | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWZ1415 | Flamma® 581 Azide | 581 | 596 | Alexa594, DyLight594 |
| PWZ1215 | Flamma® 648 Azide | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWZ1515 | Flamma® 675 Azide | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWZ1301 | Flamma® 749 Azide | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWZ1603 | Flamma® 774 Azide | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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