Flamma® 648 NHS ester
Size of product (mg)
-
1 mg
-
5 mg
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25 mg
Flamma® 648 NHS ester
Cat. No. List below
Description
With excitation and emission maxima at 648 nm and 663 nm respectively, Flamma 648 is spectrally similar to popular fluorophores like Alexa 647, Cy5, and DyLight 650. It can be efficiently excited using 593 or 633 nm laser lines, offering excellent compatibility with a wide range of imaging systems.
Key Features:
1. Far-red fluorescence with Ex/Em maxima at 648/663 nm
2. High extinction coefficient (≥ 237,500 cm?¹M?¹)
3. Excellent photostability and optical properties
4. Efficient conjugation to biomolecules
5. High molar ratios for enhanced signal strength
6. Low CF280 value (0.03) for minimal interference with protein absorbance
Applications:
1. Fluorescence microscopy
2. Flow cytometry
3. In vivo imaging
4. Protein and nucleic acid labeling
5. Cellular tracking and detection
6. Labeling of antibodies, peptides, proteins, and ligands
7. Amplification substrate optimization
Advantages:
1. High sensitivity for detecting low-abundance biomolecules
2. Stable and long-lasting fluorescence signal
3. Versatile labeling capabilities for various biomolecules
4. Ideal for deep-tissue and whole-body imaging due to far-red spectrum
5. Reduced autofluorescence and phototoxicity in biological samples
6. Compatible with common far-red laser excitation sources
The NHS ester reactive group of Flamma 648 readily forms stable amide bonds with primary amines, including the ε-amino groups of lysine residues in proteins and the amine terminus of nucleotides or modified oligonucleotides. This chemistry ensures efficient and durable labeling of target biomolecules.
- Fluorophore: Flamma® Fluors 648
- Reactive group: NHS ester
- Excitation/Emission Max.(nm): 648/663
- Spectrally similar dyes: Alexa647, DyLight650, Cy5
- Extinction coefficient: ≥ 237,500 cm-1M-1
- CF280: 0.03
- Appearance: Red Solid
- Molecular Weight: 781.93 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light, void repeated freeze-thaw cycles
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWS1001 | Flamma® 496 NHS ester | 496 | 520 | Alexa488, FITC, Cy2 |
| PWS1122 | Flamma® 552 NHS ester | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWS1415 | Flamma® 581 NHS ester | 581 | 596 | Alexa594, DyLight594 |
| PWS1215 | Flamma® 648 NHS ester | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWS1515 | Flamma® 675 NHS ester | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWS1301 | Flamma® 749 NHS ester | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWS1603 | Flamma® 774 NHS ester | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Superior Brightness: High extinction coefficient for enhanced sensitivity
Stability: Generates stable fluorescence signals for reliable results
Versatility: Compatible with various biomolecules and applications
Ease of Use: Ready-to-use format for straightforward labeling procedures
Flamma® Fluors NHS ester / Sulfo-NHS ester
Succinimidyl (NHS) esters are reliable activating moiety for amine labeling due to the formation of the strong amide bond. NHS esters are generally stable when storing in anhydrous and the dark condition at -20. NHS ester dyes display good reactivity toward aliphatic amines yet low reactivity with aromatic amines, alcohols, phenols and histidine. The basicity of an amine is an important factor for its reactivity: virtually all proteins have lysine residues, and most of them have a free N-terminus amine. The reactivity of ε-amino group at lysine is pH dependent that the optimal pH for labeling lysine residue is 8.5~9.5. However, the primary amine at N-terminus usually has a pKa value of ~7, thus it can be selectively conjugated by running the reaction in neutral pH. Due to the solubility issue, Flamma® Fluors NHS esters are required to be dissolved in an organic solvent such as DMSO or DMF and added to aqueous solution. To overcome this limitation, BioActs offers water soluble Flamma® Fluors Sulfo-NHS ester dyes, which can eliminate the usage of organic solvents in the conjugation reaction. NHS and Sulfo-NHS ester dyes are unstable under humid condition, thus they should be in a lyophilized form or a solution in the anhydrous organic solvent such as DMF or DMSO and stored at -20 ℃.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse
Citation & Reference
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