Flamma® 496 Azide
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 496 Azide
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 496/520 nm
2. High extinction coefficient: ≥ 50,000 cm?¹M?¹
3. Low CF280: 0.12
4. Azide reactive group connected through an amino propyl linkage
5. Yellow solid appearance
6. Molecular weight: 495.42 g/mol
7. Soluble in DMF and DMSO
Applications:
1. Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reactions
2. Cellular imaging and detection
3. Nucleotide functionalization
4. Biomolecule labeling and tracking
5. Live-cell imaging studies
6. Fluorescence microscopy
7. Flow cytometry
8. Metabolic labeling
9. Peptide and protein engineering
10. Chemical biology
11. Surface functionalization
Advantages:
1. Spectral similarity to popular dyes (Alexa488, FITC, Cy2)
2. Excellent optical properties when excited with a 488 nm laser line
3. Versatile click chemistry applications through CuAAC reactions
4. Stable fluorescence signal for extended imaging sessions
5. High sensitivity for detecting low-abundance biological structures
6. Minimal interference with native biochemical processes
7. Bioorthogonal labeling strategy
8. Compatibility with living systems
9. Fast reaction kinetics and high efficiency
- Fluorophore: Flamma® Fluors 496
- Reactive group: Azide
- Excitation/Emission Max.(nm): 496/520
- Spectrally similar dyes: Alexa488, FITC, Cy2
- Extinction coefficient: ≥ 50,000 cm-1M-1
- CF280: 0.12
- Appearance: Yellow Solid
- Molecular Weight: 495.42 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWZ1001 | Flamma® 496 Azide | 496 | 520 | Alexa488, FITC, Cy2 |
| PWZ1122 | Flamma® 552 Azide | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWZ1415 | Flamma® 581 Azide | 581 | 596 | Alexa594, DyLight594 |
| PWZ1215 | Flamma® 648 Azide | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWZ1515 | Flamma® 675 Azide | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWZ1301 | Flamma® 749 Azide | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWZ1603 | Flamma® 774 Azide | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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