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Flamma® 648 Azide

Product#: PWZ1215

$1,100.80

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Flamma® 648 Azide

Cat. No. List below

Description

Flamma® Fluors 648 Azide is a state-of-the-art far-red fluorescent dye specifically engineered for copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reactions in bioimaging applications. This innovative reagent combines the power of click chemistry with exceptional fluorescence properties, offering researchers a versatile and efficient tool for advanced cellular imaging and biomolecule labeling.

Key Features and Advantages:

Optimal Spectral Properties: Excitation/emission maxima at 648/663 nm, comparable to popular dyes like Alexa 647, Cy5, and DyLight 650.
Versatile Excitation: Compatible with 593 or 633 nm laser lines, providing flexibility in imaging setups.
Superior Brightness: High extinction coefficient of ≥ 227,000 cm-1M-1 ensures vivid fluorescence signals.
Minimal Protein Interference: Low CF280 value of 0.023 reduces interference with protein absorbance measurements.
Strategic Azide Positioning: Azide group connected via an amino propyl linkage for optimal reactivity.
Bioorthogonal Labeling: Forms 1,4-disubstituted 1,2,3-triazoles without disrupting native biochemical processes.

Flamma® Fluors 648 Azide excels in various applications, including advanced cellular imaging (ideal for high-resolution live-cell and fixed-cell microscopy), nucleotide functionalization (enables specific labeling of alkyne-modified nucleic acids), protein labeling (excellent for tracking alkyne-modified proteins in complex biological systems) and biomolecule tracking (suitable for studying dynamics of various alkyne-tagged biomolecules in living systems).

The Click Chemistry Advantage:
The azide reactive group allows for highly specific and efficient labeling through CuAAC reactions:

Bioorthogonal: Reactions occur without interfering with native biological processes.
High Specificity: Selective labeling of alkyne-modified biomolecules.
Versatility: Compatible with a wide range of alkyne-functionalized targets.
Mild Reaction Conditions: Suitable for various biological systems and pH ranges.

For best results. introduce alkyne functionality to target biomolecules via chemical or genetic modification, perform CuAAC reaction under optimized conditions (e.g., copper catalyst, reducing agent, ligand), purify labeled biomolecules using standard chromatographic techniques, and verify labeling efficiency through spectrophotometric analysis.

Specifications

Fluorophore: Flamma® Fluors 648
Reactive group: Azide
Excitation/Emission Max.(nm): 648/663
Spectrally similar dyes: Alexa647, DyLight650, Cy5
Extinction coefficient: ≥ 227,000 cm-1M-1
CF280: 0.023
Appearance: Blue Solid
Molecular Weight: 766.97 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
CWZ1001	Flamma® 496 Azide	496	520	Alexa488, FITC, Cy2
PWZ1122	Flamma® 552 Azide	550	564	Alexa555, DyLight549, Cy3, ATTO550
KWZ1415	Flamma® 581 Azide	581	596	Alexa594, DyLight594
PWZ1215	Flamma® 648 Azide	648	663	Alexa647, DyLight650, Cy5
PWZ1515	Flamma® 675 Azide	674	691	Alexa680, DyLight680, Cy5.5, IRDye680LT
PWZ1301	Flamma® 749 Azide	749	774	Alexa750, DyLight755, Cy7.5, IRDye750
PWZ1603	Flamma® 774 Azide	774	800	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

Why Choose Flamma® Fluors 648 Azide?

Cutting-Edge Technology: Combines click chemistry with far-red fluorescence for advanced bioimaging.
Exceptional Brightness: High extinction coefficient for clear, vivid signals even at low concentrations.
Versatility: Suitable for various alkyne-modified biomolecules and imaging techniques.
Minimal Background: Low CF280 value ensures high signal-to-noise ratio in protein studies.
Optimized Design: Amino propyl linkage provides ideal spacing for efficient click reactions.

Flamma^® Fluors for Click Chemistry

The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma^® Fluors Alkyne dyes for CuAAC, Flamma^® Fluors ADIBO products for SPAAC and Flamma^® Fluors Azide dyes for both CuAAC and SPAAC.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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