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TAMRA Alkyne

Product#: KWK1025

$797.28

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5 mg
25 mg

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TAMRA Alkyne

Cat. No. List below

Description

TAMRA Alkyne is a versatile copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent that generates a stable, bright yellow-orange fluorescence signal for bioimaging applications. This click chemistry-compatible fluorophore offers excellent optical properties and is widely used in cellular imaging and nucleotide functionalization.

Key Features:
1. Bright yellow-orange fluorescence
2. Excitation/Emission maxima: 543/575 nm
3. Extinction coefficient: ≥ 23,000 cm?¹M?¹
4. Molecular Weight: 469.53 g/mol
5. Appearance: Red solid
6. Solubility: DMF
7. Storage: -20°C, protected from light

Applications:
1. Cellular imaging
2. Nucleotide functionalization
3. Protein labeling
4. Biomolecule tracking in living systems
5. Flow cytometry
6. Fluorescence microscopy
7. Single-molecule detection
8. DNA synthesis detection

Advantages:
1. Efficient excitation with 543 or 546 nm laser lines
2. Spectrally similar to popular dyes like DyLight 549, ATTO 550, and Cy3
3. Forms stable 1,4-disubstituted 1,2,3-triazole linkages
4. Minimal interference with native biochemical processes
5. Bioorthogonal labeling strategy
6. High specificity and efficiency in click reactions
7. Versatile labeling of azide-modified biomolecules
8. Compatible with complex biological samples

TAMRA Alkyne couples with azide-functionalized biomolecules through copper(I)-catalyzed click chemistry, forming a stable 1,4-disubstituted 1,2,3-triazole linkage. This reaction occurs efficiently inside living systems without disrupting native biochemical processes. To utilize TAMRA Alkyne, researchers must first introduce azide functionality onto the target biomolecule through chemical or genetic modification.

The CuAAC reaction mechanism involves multiple steps, including the formation of a copper-acetylide complex, coordination of the azide to the copper center, and subsequent cycloaddition to form the triazole product. Recent studies have shown that the reaction proceeds faster in aqueous conditions and may involve a dinuclear copper intermediate.

Specifications

Fluorophore: TAMRA
Reactive group: Alkyne
Excitation/Emission Max.(nm): 543/575
Spectrally similar dyes: DyLight549, Cy3, ATTO550
Extinction coefficient: ≥ 23,000 cm-1M-1
Appearance: Red Solid
Molecular Weight: 469.53 g/mol
Solubility: DMF
Storage conditions: -20 ℃, protect from light

TAMRA Dyes

Quick link (Cat.#)	Series	Quick link (Cat.#)	Series
KWS1025	TAMRA NHS ester	KWSN1025	TAMRA Sulfo-NHS ester
KWA1020	TAMRA Vinylsulfone	KWM1057	TAMRA Maleimide
KWZ1025	TAMRA Azide	KWH1025	TAMRA Hydrazide
KWK1025	TAMRA Alkyne	KWG1025	TAMRA PEG4-Alkyne
DWR1001	TAMRA ADIBO	KWE1025	TAMRA Amine
KWT1057	TAMRA Thiol	KWR2025	TAMRA Dichlorotriazine

Background

Other Labeling Dyes

BioActs provides other traditionally used dyes such as 5(6)-Carboxyfluorescein (FAM) and 5(6)-Carboxytetramethylrhodamine (TAMRA) dyes for labeling of biomolecules. FAM is one of popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. In addition to relatively high absorptivity, good fluorescence quantum yield and good water solubility, FAM has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. TAMRA fluorophore has been a widely used for preparing bioconjugates, especially fluorescent antibody and avidin derivatives. TAMRA dye is also widely utilized for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore.

Other fluorescent dyes such as Cyanine, ICG, TAMRA, FAM, etc. are also available.
All dyes are equipped with various reactive and functional groups.
High quality and excellent performance

Table 1. TAMRA dye applications

Figure 1. Structure of FAM and TAMRA dyes

Citation & Reference

1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.

2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.

3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).

4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.

5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.