FSD Fluor™ 647 NHS ester

Product#: KOSC1315

$3,943.36

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FSD Fluor™ 647 NHS ester

Cat. No. List below

Description

FSD Fluor™ 647 NHS ester is a next-generation, far-red fluorescent dye developed by BioActs. It offers superior optical properties compared to spectrally similar dyes, making it ideal for various biochemical and biological analytical applications.

FSD Fluor™ 647 NHS ester is optimized for labeling antibodies, peptides, proteins, ligands, and amplification substrates. These applications are particularly suited for cellular labeling and detection.

Elevate your fluorescence-based experiments with FSD Fluor™ 647 NHS ester and experience the difference in brightness, stability, and overall performance that only next-generation fluorophore technology can deliver. NHS esters readily react with amine-modified oligonucleotides or amino groups of proteins, i.e. the ε-amino groups of lysine or the amine terminus of nucleotides to form a chemically stable amide bond between dye and the biomolecule.

What are the advantages?
1. High Fluorescence Intensity: Excellent brightness after binding to biomolecules such as antibodies, nucleotides, and proteins.
2. Photostability: Enhanced stability for reliable and long-lasting fluorescence signals.
3. Single-Molecule Detection: Ideal for fluorescence correlation spectroscopy and fluorescence polarization measurements.
4. Reactivity: NHS esters readily react with amine-modified oligonucleotides and amino groups of proteins to form stable amide bonds.

Elevate your fluorescence-based experiments with FSD Fluor™ 647 NHS ester and experience the difference in brightness, stability, and overall performance that only next-generation fluorophore technology can deliver.

FSD Fluor™ 647 NHS ester offers superior fluorescence intensity compared to Alexa647 and DyLight650:

Feature	FSD Fluor™ 647	Alexa647 / DyLight650
Fluorescence Intensity	Up to 30% higher	Standard
Quantum Yield	Excellent	Good
Photostability	Enhanced	Standard
Low-Abundance Target Sensing	Higher sensitivity	Standard
Signal-to-Noise Ratio	Improved	Standard
Overall Performance	Next-generation performance	Well-established

Specifications

Fluorophore: FSD Fluor™ 647
Reactive group: NHS ester
Excitation/Emission Max.(nm): 651/667
Spectrally similar dyes: Alexa647, DyLight650, Cy5
Extinction coefficient: ≥ 217,000 cm-1M-1
CF280: 0.03
Appearance: Blue Liquid
Molecular Weight: 1254.86 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm	Spectrally similar dyes
KOSC1002	FSD Fluor™ 488	495	519	Alexa Fluor 488, FAM, Cy2
KOSC1003	FSD Fluor™ 555	554	565	Alexa Fluor 555, DyLight 549, Cy3, ATTO 550
KOSC1001	FSD Fluor™ 594	591	617	Alexa Fluor 594, DyLight 594
KOSC1315	FSD Fluor™ 647	651	667	Alexa Fluor 647, Cy5, ATTO 647N, DyLight 650
KOSC1515	FSD Fluor™ 680	679	696	Alexa Fluor 680, Cy5.5, DyLight 680, IRDye 680
KOSC1702	FSD Fluor™ 750	749	774	Alexa Fluor 750, DyLight 755, Cy7, IRDye 750
POSC1803	FSD Fluor™ 800	774	790	Cy7.5, DyLight 800, IRDye 800

Background
FSD Fluor™
FSD Fluor™ is a new generation of dye series with superb fluorescence intensity and high quantum yield comparing to traditional dyes. The fluorescence intensity after binding to biomolecules such as antibody, nucleotide, and protein maintains still excellent, FSD Fluor™ series is ideal for variety of biochemical and biological analytical applications with a less amount of dye conjugate. FSD Fluor™ series equipped with a variety of reactive groups and covers the full fluorescence spectral range from UV to NIR, hence the series is ideal for any applications in fluorescence spectroscopies and biological studies. With superior fluorophores and the wide spectral range, FSD Fluor™ dyes are suitable for every filter and are designed to meet the requirements for complex detection in the field of life science research.

Key Benefits:

Superior brightness: up to 30% brighter than leading competitors, enabling detection fo low-abundance targets.
Excellent Stability: Maintains high fluorescence intensity after bioconjugation
Versatile Applications: Ideal for antibody labeling, nucleic acid detection, and protein studies
Optimized for Single-Molecule Detection: Perfect for fluorescence correlation spectroscopy and polarization measurements
Higher quantum yield comparing to traditional dyes
Equipped with variety of reactive group
Covering the full fluorescence spectral range from visible to NIR
Enhanced Sensitivity: Detect targets with greater accuracy, even at low concentrations
Reduced Background: Higher signal-to-noise ratio for clearer images and more reliable data
Cost-Effective: Achieve superior results with less dye, maximizing your research budget

Figure 1. Fluorescence intensity comparison of FSD Fluor™ and other dyes

Figure 2. Fluorescence intensity comparison in varying dye/protein ratio

Figure 3. Immunofluorescence comparison of dye-antibody conjugates and photostability test of fluorescent secondary antibodies

Figure 4. Biodistribution images of FSD Fluor™ 750 and FSD Fluor™ 800

Citation & Reference

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