FSD Fluor™ 555 NHS ester

Product#: KOSC1003

$3,943.36

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FSD Fluor™ 555 NHS ester

Cat. No. List below

Description

FSD Fluor™ 555 NHS ester is a state-of-the-art, amine-reactive fluorescent dye developed by BioActs, offering exceptional optical properties for a wide range of biochemical and biological analytical applications. This next-generation bright yellow dye is engineered to surpass the performance of spectrally similar fluorophores, making it an ideal choice for researchers demanding high sensitivity and superior fluorescence intensity in their experiments.

FSD Fluor™ 555 NHS ester readily reacts with amine-modified oligonucleotides, proteins (ε-amino groups of lysine or N-terminus), peptides, antibodies, ligands, and amplification substrates.

It has excellent fluorescence intensity after biomolecule conjugation. It is ideal for single-molecule detection in fluorescence correlation spectroscopy and optimal for fluorescence polarization measurements. It hsd high sensitivity for low-abundance biomolecule detection. It forms stable amide bonds with target molecules, ensuring long-lasting labeling.

Unique Advantages:
1. Enhanced Brightness: Outperforms spectrally similar dyes in fluorescence intensity
2. High Molar Ratio Labeling: Allows for sensitive detection of low-abundance targets
3. Versatility: Suitable for a wide range of biomolecules and applications
4. Stability: Resistant to photobleaching, enabling extended imaging sessions
5. Compatibility: Spectral properties similar to widely-used dyes, allowing easy integration into existing protocols
6. High Extinction Coefficient: Ensures bright fluorescence signals even at low concentrations

FSD Fluor™ 555 NHS ester represents a significant advancement in fluorescent labeling technology, offering researchers a powerful tool for exploring biological systems with unprecedented sensitivity and clarity. Its combination of bright fluorescence, high photostability, and versatile reactivity makes it an invaluable asset in modern biomedical research, particularly for applications requiring high sensitivity, resolution, and multiplexing capabilities.

Specifications

Fluorophore: FSD Fluor™ 555
Reactive group: NHS ester
Excitation/Emission Max.(nm): 554/565
Spectrally similar dyes: Alexa555, DyLight549, Cy3, ATTO550
Extinction coefficient: ≥ 141,000 cm-1M-1
CF280: 0.08
Appearance: Red Liquid
Molecular Weight: 1228.82 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm	Spectrally similar dyes
KOSC1002	FSD Fluor™ 488	495	519	Alexa Fluor 488, FAM, Cy2
KOSC1003	FSD Fluor™ 555	554	565	Alexa Fluor 555, DyLight 549, Cy3, ATTO 550
KOSC1001	FSD Fluor™ 594	591	617	Alexa Fluor 594, DyLight 594
KOSC1315	FSD Fluor™ 647	651	667	Alexa Fluor 647, Cy5, ATTO 647N, DyLight 650
KOSC1515	FSD Fluor™ 680	679	696	Alexa Fluor 680, Cy5.5, DyLight 680, IRDye 680
KOSC1702	FSD Fluor™ 750	749	774	Alexa Fluor 750, DyLight 755, Cy7, IRDye 750
POSC1803	FSD Fluor™ 800	774	790	Cy7.5, DyLight 800, IRDye 800

Background

FSD Fluor™

FSD Fluor™ is a new generation of dye series with superb fluorescence intensity and high quantum yield comparing to traditional dyes. The fluorescence intensity after binding to biomolecules such as antibody, nucleotide, and protein maintains still excellent, FSD Fluor™ series is ideal for variety of biochemical and biological analytical applications with a less amount of dye conjugate. FSD Fluor™ series equipped with a variety of reactive groups and covers the full fluorescence spectral range from UV to NIR, hence the series is ideal for any applications in fluorescence spectroscopies and biological studies. With superior fluorophores and the wide spectral range, FSD Fluor™ dyes are suitable for every filter and are designed to meet the requirements for complex detection in the field of life science research.

Superior fluorescence intensity than any other spectrally similar dyes
Maintaining excellent fluorescence after conjugated to biomolecules, surpassing that of any other spectrally similar fluorophore
conjugated biomolecules.
Higher quantum yield comparing to traditional dyes
Equipped with variety of reactive group
Covering the full fluorescence spectral range from visible to NIR

Figure 1. Fluorescence intensity comparison of FSD Fluor™ and other dyes

Figure 2. Fluorescence intensity comparison in varying dye/protein ratio

Figure 3. Immunofluorescence comparison of dye-antibody conjugates and photostability test of fluorescent secondary antibodies

Figure 4. Biodistribution images of FSD Fluor™ 750 and FSD Fluor™ 800

Citation & Reference

1. Xu, Peisheng. Zwitterionic chitosan derivatives for pH-sensitive stealth coating. Biomacromolecules 11.9 (2010): 2352-2358.
2. Ibrahim, Basma M. A strategy to deliver genes to cystic fibrosis lungs: a battle with environment. Journal of controlled release 155.2 (2011): 289-295.
3. Oh, Keun Sang. Accurate sequential detection of primary tumor and metastatic lymphatics using a temperature-induced phase transition nanoparticulate system. International journal of nanomedicine 9 (2014): 2955.
4. Yhee, Ji Young. Tumor-targeting transferrin nanoparticles for systemic polymerized siRNA delivery in tumor-bearing mice. Bioconjugate chemistry 24.11 (2013): 1850-1860.
5. Yoon, Hong Yeol. Glycol chitosan nanoparticles as specialized cancer therapeutic vehicles: Sequential delivery of doxorubicin and Bcl-2 siRNA. Scientific reports 4 (2014).
6. Ryu, Ju Hee. Early diagnosis of arthritis in mice with collagen?induced arthritis, using a fluorogenic matrix metalloproteinase 3–specific polymeric probe. Arthritis & Rheumatism 63.12 (2011): 3824-3832.
7. Hollis, Christin P. In vivo investigation of hybrid paclitaxel nanocrystals with dual fluorescent probes for cancer theranostics. Pharmaceutical research 31.6 (2014): 1450-1459.
8. Koo, Heebeom. The movement of self-assembled amphiphilic polymeric nanoparticles in the vitreous and retina after intravitreal injection. Biomaterials 33.12 (2012): 3485-3493.
9. Zhu, Lei. Real-time monitoring of caspase cascade activation in living cells. Journal of controlled release 163.1 (2012): 55-62.
10. Yoon, Hong Yeol. Bioreducible hyaluronic acid conjugates as siRNA carrier for tumor targeting. Journal of Controlled Release 172.3 (2013): 653-661.
11. Yhee, Ji Young. Cancer-targeted MDR-1 siRNA delivery using self-cross-linked glycol chitosan nanoparticles to overcome drug resistance. Journal of Controlled Release 198 (2015): 1-9.
12. Park, Jin Woo. Wide-Ranged Fluorescent Molecular Weight Size Markers for Electrophoresis. Bulletin of the Korean Chemical Society 34.1 (2013): 29-30.
13. Huang, Xinglu. Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad?Spectrum Nanoquencher. Angewandte Chemie International Edition 51.7 (2012): 1625-1630.