FSD Fluor™ 680 NHS ester

Product#: KOSC1515

$3,943.36

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FSD Fluor™ 680 NHS ester

Cat. No. List below

Description

FSD Fluor™ 680 NHS ester is a cutting-edge near-infrared (NIR) fluorescent dye that revolutionizes biological imaging and analysis. This next-generation dye offers unparalleled sensitivity and versatility, setting new standards in the field of bioimaging.

Key features of FSD Fluor™ 680 NHS ester include:
1. Superior brightness: Up to 30% brighter than leading competitors
2. Exceptional stability: Maintains high fluorescence intensity after bioconjugation
3. Enhanced sensitivity: Detects low-abundance targets with unprecedented clarity
3. Optimal spectral properties: Excitation/Emission maxima at 679/696 nm
5. Compatibility: Similar spectral characteristics to Alexa 680, Cy5.5, IRDye 680LT, and DyLight 680
6. Versatile labeling: Efficiently conjugates to proteins, peptides, antibodies, and nucleotides.

Applications of FSD Fluor™ 680 NHS ester encompass:
1. In vivo imaging with deep tissue penetration
2. High-resolution flow cytometry
3. Advanced fluorescence microscopy
4. Single-molecule detection in fluorescence correlation spectroscopy
5. Precise fluorescence polarization measurements
6. Multiplexed western blot detection

The dye's NIR properties (679/696 nm Ex/Em) allow for imaging with minimal autofluorescence from biological samples, reduced light scattering, and high tissue penetration6. It can be excited using the 633 nm laser line, and its emission occurs in the biologically permeable NIR region.
FSD Fluor™ 680 NHS ester forms stable amide bonds with amino groups of proteins (e.g., ε-amino groups of lysine) and amine-modified nucleotides, ensuring reliable and long-lasting labeling. This property makes it ideal for labeling low-abundance biomolecules with high sensitivity.
With its exceptional performance in various biochemical and biological analytical applications, FSD Fluor™ 680 NHS ester is poised to become an indispensable tool for researchers in fields such as cancer detection, tumor resection, and advanced bioimaging techniques.

Specifications

Fluorophore: FSD Fluor™ 680
Reactive group: NHS ester
Excitation/Emission Max.(nm): 679/696
Spectrally similar dyes: Alexa 680, DyLight 680, Cy 5.5, IRDye 680LT
Extinction coefficient: ≥ 200,000 cm-1M-1
CF280: 0.09
Appearance: Blue Liquid
Molecular Weight: 1515.1 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm	Spectrally similar dyes
KOSC1002	FSD Fluor™ 488	495	519	Alexa Fluor 488, FAM, Cy2
KOSC1003	FSD Fluor™ 555	554	565	Alexa Fluor 555, DyLight 549, Cy3, ATTO 550
KOSC1001	FSD Fluor™ 594	591	617	Alexa Fluor 594, DyLight 594
KOSC1315	FSD Fluor™ 647	651	667	Alexa Fluor 647, Cy5, ATTO 647N, DyLight 650
KOSC1515	FSD Fluor™ 680	679	696	Alexa Fluor 680, Cy5.5, DyLight 680, IRDye 680
KOSC1702	FSD Fluor™ 750	749	774	Alexa Fluor 750, DyLight 755, Cy7, IRDye 750
POSC1803	FSD Fluor™ 800	774	790	Cy7.5, DyLight 800, IRDye 800

Background

FSD Fluor™ is a new generation of dye series with superb fluorescence intensity and high quantum yield comparing to traditional dyes. The fluorescence intensity after binding to biomolecules such as antibody, nucleotide, and protein maintains still excellent, FSD Fluor™ series is ideal for variety of biochemical and biological analytical applications with a less amount of dye conjugate. FSD Fluor™ series equipped with a variety of reactive groups and covers the full fluorescence spectral range from UV to NIR, hence the series is ideal for any applications in fluorescence spectroscopies and biological studies. With superior fluorophores and the wide spectral range, FSD Fluor™ dyes are suitable for every filter and are designed to meet the requirements for complex detection in the field of life science research.

Key Benefits:

Superior brightness: up to 30% brighter than leading competitors, enabling detection fo low-abundance targets.
Excellent Stability: Maintains high fluorescence intensity after bioconjugation
Versatile Applications: Ideal for antibody labeling, nucleic acid detection, and protein studies
Optimized for Single-Molecule Detection: Perfect for fluorescence correlation spectroscopy and polarization measurements
Higher quantum yield comparing to traditional dyes
Equipped with variety of reactive group
Covering the full fluorescence spectral range from visible to NIR
Enhanced Sensitivity: Detect targets with greater accuracy, even at low concentrations
Reduced Background: Higher signal-to-noise ratio for clearer images and more reliable data
Cost-Effective: Achieve superior results with less dye, maximizing your research budget

Why Choose FSD Fluor™ 680?

Versatile Conjugation: Easily label antibodies, peptides, proteins, and nucleic acids
Tissue-Permeable NIR Emission: Ideal for in vivo and deep-tissue imaging
Low Background: Higher signal-to-noise ratio for clearer data
Cost-Effective: Achieve superior results with less dye

Figure 1. Fluorescence intensity comparison of FSD Fluor™ and other dyes

Figure 2. Fluorescence intensity comparison in varying dye/protein ratio

Figure 3. Immunofluorescence comparison of dye-antibody conjugates and photostability test of fluorescent secondary antibodies

Figure 4. Biodistribution images of FSD Fluor™ 750 and FSD Fluor™ 800

Citation & Reference

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