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TAMRA Thiol

Product#: KWT1057

$839.84

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5 mg
25 mg

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TAMRA Thiol

Cat. No. List below

Description

TAMRA Thiol is an inactive form of bright yellow fluorescent dye used to generate stable fluorescence signals in bioimaging applications. This versatile fluorophore offers excellent optical properties and is widely used in various biological and biochemical studies.

Key Features:
1. Bright yellow-orange fluorescence
2. Excitation/Emission maxima: 543/575 nm
3. Extinction coefficient: ≥ 70,000 cm?¹M?¹
4. Molecular Weight: 491.60 g/mol
5. Appearance: Red solid
6. Solubility: DMF, DMSO
7. Storage: -20°C, protected from light
8. Thiol group attached through an aminoethyl linkage
9. CF280: 0.21

Applications:
1. Bioimaging and fluorescence microscopy
2. Protein and peptide labeling
3. Nucleotide functionalization
4. Flow cytometry
5. Fluorescence in situ hybridization (FISH)
6. Reference standard for dye-conjugates
7. Thiol-specific labeling in live cells and tissues

Advantages:
1. Efficient excitation with 543 or 546 nm laser lines
2. Spectrally similar to popular dyes like DyLight 549, ATTO 550, and Cy3
3. Forms stable 1,4-disubstituted 1,2,3-triazole linkages
4. Minimal interference with native biochemical processes
5. High sensitivity for detecting biothiols in biological samples
6. Suitable for live-cell imaging and in vivo applications
7. Low cytotoxicity at commonly used concentrations
8. Versatile labeling of thiol-containing biomolecules

TAMRA Thiol can be labeled to biomolecules through disulfide bond formation with thiol groups of cysteine residues. This property makes it particularly useful for studying proteins, peptides, and other biomolecules containing accessible cysteine residues. The thiol functional group also allows for the detection and quantification of biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) in biological samples.

Specifications

Fluorophore: TAMRA
Functional group: Thiol
Excitation/Emission Max.(nm): 553/575
Spectrally similar dyes: DyLight549, Cy3, ATTO550
Extinction coefficient: ≥ 70,000 cm-1M-1
CF280: 0.21
Appearance: Red Solid
Molecular Weight: 491.60 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

TAMRA Dyes

Quick link (Cat.#)	Series	Quick link (Cat.#)	Series
KWS1025	TAMRA NHS ester	KWSN1025	TAMRA Sulfo-NHS ester
KWA1020	TAMRA Vinylsulfone	KWM1057	TAMRA Maleimide
KWZ1025	TAMRA Azide	KWH1025	TAMRA Hydrazide
KWK1025	TAMRA Alkyne	KWG1025	TAMRA PEG4-Alkyne
DWR1001	TAMRA ADIBO	KWE1025	TAMRA Amine
KWT1057	TAMRA Thiol	KWR2025	TAMRA Dichlorotriazine

Background

Other Labeling Dyes

BioActs provides other traditionally used dyes such as 5(6)-Carboxyfluorescein (FAM) and 5(6)-Carboxytetramethylrhodamine (TAMRA) dyes for labeling of biomolecules. FAM is one of popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. In addition to relatively high absorptivity, good fluorescence quantum yield and good water solubility, FAM has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. TAMRA fluorophore has been a widely used for preparing bioconjugates, especially fluorescent antibody and avidin derivatives. TAMRA dye is also widely utilized for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore.

Other fluorescent dyes such as Cyanine, ICG, TAMRA, FAM, etc. are also available.
All dyes are equipped with various reactive and functional groups.
High quality and excellent performance

Table 1. TAMRA dye applications

Figure 1. Structure of FAM and TAMRA dyes

Citation & Reference

1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.

2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.

3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).

4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.

5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.