TAMRA Maleimide
Size of product (mg)
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1 mg
-
5 mg
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25 mg
TAMRA Maleimide
Cat. No. List below
Description
TAMRA Maleimide is a thiol reactive bright yellow dye that used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 553/575 nm, similar to that of DyLight 549, ATTO 550 and Cy 3. TAMRA might be excited using 543 or 546 nm laser line and displays good optical property. Maleimides selectively label thiols of cysteine residue via 1,4-addition pathway, without interacting with amines, to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they might react with primary amines under strong basic environment. We offer TAMRA maleimide for labeling of antibodies, peptides, proteins and oligonucleotide labeling and automated DNA sequencing applications.
- Fluorophore: TAMRA
- Reactive group: Maleimide
- Excitation/Emission Max.(nm): 553/575
- Spectrally similar dyes: DyLight549, Cy3, ATTO550
- Extinction coefficient: ≥ 54,000 cm-1M-1
- CF280: 0.2
- Appearance: Red Solid
- Molecular Weight: 554.60 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | Series | Quick link (Cat.#) | Series |
| KWS1025 | TAMRA NHS ester | KWSN1025 | TAMRA Sulfo-NHS ester |
| KWA1020 | TAMRA Vinylsulfone | KWM1057 | TAMRA Maleimide |
| KWZ1025 | TAMRA Azide | KWH1025 | TAMRA Hydrazide |
| KWK1025 | TAMRA Alkyne | KWG1025 | TAMRA PEG4-Alkyne |
| DWR1001 | TAMRA ADIBO | KWE1025 | TAMRA Amine |
| KWT1057 | TAMRA Thiol | KWR2025 | TAMRA Dichlorotriazine |
Background
Other Labeling Dyes
BioActs provides other traditionally used dyes such as 5(6)-Carboxyfluorescein (FAM) and 5(6)-Carboxytetramethylrhodamine (TAMRA) dyes for labeling of biomolecules. FAM is one of popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. In addition to relatively high absorptivity, good fluorescence quantum yield and good water solubility, FAM has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. TAMRA fluorophore has been a widely used for preparing bioconjugates, especially fluorescent antibody and avidin derivatives. TAMRA dye is also widely utilized for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore.
- Other fluorescent dyes such as Cyanine, ICG, TAMRA, FAM, etc. are also available.
- All dyes are equipped with various reactive and functional groups.
- High quality and excellent performance
Table 1. TAMRA dye applications
Figure 1. Structure of FAM and TAMRA dyes
Citation & Reference
1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.
2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.
3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).
4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.
5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.
