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FAM ADIBO

Product#: DWF1001

$797.28

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5 mg
25 mg

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FAM ADIBO

Cat. No. List below

Description

FAM ADIBO is a versatile strain-promoted azide-alkyne cycloaddition (SPAAC) reagent that generates a stable, bright green fluorescence signal for bioimaging applications. This copper-free click chemistry-compatible fluorophore offers excellent optical properties and is widely used in cellular imaging and nucleotide functionalization.

Key features:
1. Bright green fluorescence
2. Excitation/Emission maxima: 494/522 nm
3. Extinction coefficient: ≥ 52,000 cm?¹M?¹
4. Molecular Weight: 636.66 g/mol
5. Appearance: Yellow solid
6. Solubility: DMF
7. Storage: -20°C, protected from light

Applications:
1. Cellular imaging
2. Nucleotide functionalization
3. Protein labeling
4. Biomolecule tracking in living systems
5. Flow cytometry
6. Fluorescence microscopy
7. Single-molecule detection

Advantages:
1. Efficient excitation with 488 nm laser line
2. Spectrally similar to popular dyes like Alexa 488 and Cy2
3. Forms stable 1,4-disubstituted 1,2,3-triazole linkages
4. No coupling reagents or catalysts required
5. Minimal interference with native biochemical processes
6. Bioorthogonal labeling strategy
7. High specificity and efficiency in click reactions
8. Versatile labeling of azide-modified biomolecules
9. Compatible with complex biological samples
10. Suitable for in vivo applications due to copper-free chemistry

FAM ADIBO couples with azide-functionalized biomolecules through strain-promoted azide-alkyne cycloaddition, forming a stable 1,4-disubstituted 1,2,3-triazole linkage. This reaction occurs efficiently inside living systems without the need for coupling reagents or catalysts, making it ideal for in vivo applications. To utilize FAM ADIBO, researchers must first introduce azide functionality onto the target biomolecule through chemical or genetic modification.

The SPAAC approach offers several advantages over traditional labeling methods and copper-catalyzed click chemistry. It provides high specificity, efficiency, and bioorthogonality, allowing for sensitive detection in complex biological samples with minimal background signal. The reaction proceeds under physiological conditions without the need for potentially toxic copper catalysts, making it highly suitable for live-cell imaging and in vivo studies.

Specifications

Fluorophore: FAM
Reactive group: Strained azide
Excitation/Emission Max.(nm): 494/522
Spectrally similar dyes: Alexa488, Cy2
Extinction coefficient: ≥ 52,000 cm-1M-1
Appearance: Yellow Solid
Molecular Weight: 636.66 g/mol
Solubility: DMF
Storage conditions: -20 ℃, protect from light

FAM Dyes

Quick link (Cat.#)	Series	Quick link (Cat.#)	Series
CWS1025	FAM NHS ester	CWSN1025	FAM Sulfo-NHS ester
CWA1020	FAM Vinylsulfone	CWM1003	FAM Maleimide
CWZ1003	FAM Azide	CWH1003	FAM Hydrazide
CWK1003	FAM Alkyne	PWK1701	FAM PEG4-Alkyne
DWF1001	FAM ADIBO	CWE1003	FAM Amine
CWT1003	FAM Thiol	CWR2003	FAM Dichlorotriazine

Background

Other Labeling Dyes

BioActs provides other traditionally used dyes such as 5(6)-Carboxyfluorescein (FAM) and 5(6)-Carboxytetramethylrhodamine (TAMRA) dyes for labeling of biomolecules. FAM is one of popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. In addition to relatively high absorptivity, good fluorescence quantum yield and good water solubility, FAM has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. TAMRA fluorophore has been a widely used for preparing bioconjugates, especially fluorescent antibody and avidin derivatives. TAMRA dye is also widely utilized for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore.

Other fluorescent dyes such as Cyanine, ICG, TAMRA, FAM, etc. are also available.
All dyes are equipped with various reactive and functional groups.
High quality and excellent performance

Table 1. FAM dye applications

Figure 1. Structure of FAM and TAMRA dyes

Citation & Reference

1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.

2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.

3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).

4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.

5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.