FAM Thiol
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
FAM Thiol
Cat. No. List below
Description
Key features:
1. Bright green fluorescence
2. Excitation/Emission maxima: 494/522 nm
3. Extinction coefficient: ≥ 60,000 cm?¹M?¹
4. Molecular Weight: 437.47 g/mol
5. Appearance: Yellow solid
6. Solubility: DMF, DMSO
7. Storage: -20°C, protected from light
8. CF280: 0.18
Applications:
1. Bioimaging and fluorescence microscopy
2. Protein and peptide labeling
3. Nucleotide functionalization
4. Flow cytometry
5. Fluorescence in situ hybridization (FISH)
6. Reference standard for dye-conjugates
7. Thiol-specific labeling in live cells and tissues
Advantages:
1. Efficient excitation with 488 nm laser line
2. Spectrally similar to popular dyes like Alexa 488 and Cy2
3. Forms stable disulfide bonds with cysteine residues
4. High sensitivity for detecting biothiols in biological samples
5. Suitable for live-cell imaging and in vivo applications
6. Low cytotoxicity at commonly used concentrations
7. Versatile labeling of thiol-containing biomolecules
8. Can be used to monitor changes in biothiol levels associated with oxidative stress
FAM Thiol can be labeled to biomolecules through disulfide bond formation with thiol groups of cysteine residues. This property makes it particularly useful for studying proteins, peptides, and other biomolecules containing accessible cysteine residues. The thiol functional group also allows for the detection and quantification of biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) in biological samples.
- Fluorophore: FAM
- Functional group: Thiol
- Excitation/Emission Max.(nm): 494/522
- Spectrally similar dyes: Alexa488, Cy2
- Extinction coefficient: ≥ 60,000 cm-1M-1
- CF280: 0.18
- Appearance: Yellow Solid
- Molecular Weight: 437.47 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | Series | Quick link (Cat.#) | Series |
| CWS1025 | FAM NHS ester | CWSN1025 | FAM Sulfo-NHS ester |
| CWA1020 | FAM Vinylsulfone | CWM1003 | FAM Maleimide |
| CWZ1003 | FAM Azide | CWH1003 | FAM Hydrazide |
| CWK1003 | FAM Alkyne | PWK1701 | FAM PEG4-Alkyne |
| DWF1001 | FAM ADIBO | CWE1003 | FAM Amine |
| CWT1003 | FAM Thiol | CWR2003 | FAM Dichlorotriazine |
Background
Other Labeling Dyes
BioActs provides other traditionally used dyes such as 5(6)-Carboxyfluorescein (FAM) and 5(6)-Carboxytetramethylrhodamine (TAMRA) dyes for labeling of biomolecules. FAM is one of popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. In addition to relatively high absorptivity, good fluorescence quantum yield and good water solubility, FAM has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. TAMRA fluorophore has been a widely used for preparing bioconjugates, especially fluorescent antibody and avidin derivatives. TAMRA dye is also widely utilized for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore.
- Other fluorescent dyes such as Cyanine, ICG, TAMRA, FAM, etc. are also available.
- All dyes are equipped with various reactive and functional groups.
- High quality and excellent performance
Table 1. FAM dye applications
Figure 1. Structure of FAM and TAMRA dyes
Common Questions & Answers
Q: What is the approximate solubility of this compound in DMSO? Will it be ~ 10mg/ml or greater?
A: This product is indeed soluble in DMSOs and it does have a solubility of 10mg/ml and over.
Q: Would this compound react to maleimide functional group?
A: Thiol binds with maleimide.
Citation & Reference
1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.
2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.
3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).
4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.
5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.
