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FAM Vinylsulfone

Product#: CWA1020

$808.48

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1 mg
5 mg
25 mg

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FAM Vinylsulfone

Cat. No. List below

Description

FAM Vinylsulfone is a pH-insensitive reactive form of bright green fluorescent dye designed for generating stable fluorescence signals in bioimaging applications. This innovative dye, developed using BioActs' leading technology, offers exceptional stability across a wide pH range and at high temperatures, making it a versatile tool for various research applications.

Key features:
1. Bright green fluorescence
2. pH insensitivity
3. High temperature stability
4. Excitation/Emission maxima: 494/518 nm
5. Extinction coefficient: ≥ 58,000 cm?¹M?¹
6. CF280: 0.2
7. Molecular Weight: 495.5 g/mol
8. Appearance: Yellow solid
9. Solubility: DMF, DMSO
10. Storage: 4°C, protected from light

Applications:
1. Labeling of antibodies, peptides, proteins, and nucleotides
2. Fluorescence microscopy and bioimaging
3. Flow cytometry
4. Fluorescence in situ hybridization (FISH)
5. Single-molecule detection
6. Protein tracking and localization studies

Advantages:
1. Stable fluorescence signal across a wide pH range
2. High temperature resistance for versatile experimental conditions
3. Efficient excitation with 488 nm laser line
4. Spectrally similar to popular dyes like Alexa 488 and Cy2 for easy integration into existing protocols
5. Forms stable amino linkages with biomolecules through vinylsulfone chemistry
6. Suitable for harsh labeling conditions, including less reactive amines and prolonged reaction times
7. Can be used in both aqueous and organic solutions
8. Minimal side product generation during conjugation

The vinylsulfone reactive group of FAM Vinylsulfone readily reacts with primary amines of amino-modified oligonucleotides or proteins, forming a stable amino linkage between the dye and the target biomolecule. This reaction occurs efficiently under a wide range of pH conditions (pH 5-10), with the labeling rate proportional to the basicity of the medium.

FAM Vinylsulfone's unique combination of pH insensitivity, temperature stability, and efficient conjugation chemistry makes it an excellent choice for researchers seeking reliable and versatile fluorescent labeling options. Its ability to maintain performance under challenging conditions and compatibility with various biomolecules position FAM Vinylsulfone as a valuable tool for advancing fluorescence-based research across multiple disciplines in life sciences.

Specifications

Fluorophore: FAM
Reactive group: Vinylsulfone
Excitation/Emission Max.(nm): 494/518
Spectrally similar dyes: Alexa488, Cy2
Extinction coefficient: ≥ 58,000 cm-1M-1
CF280: 0.2
Appearance: Yellow Solid
Molecular Weight: 495.5 g/mol
Solubility: DMF, DMSO
Storage conditions: 4 ℃, protect from light

FAM Dyes

Quick link (Cat.#)	Series	Quick link (Cat.#)	Series
CWS1025	FAM NHS ester	CWSN1025	FAM Sulfo-NHS ester
CWA1020	FAM Vinylsulfone	CWM1003	FAM Maleimide
CWZ1003	FAM Azide	CWH1003	FAM Hydrazide
CWK1003	FAM Alkyne	PWK1701	FAM PEG4-Alkyne
DWF1001	FAM ADIBO	CWE1003	FAM Amine
CWT1003	FAM Thiol	CWR2003	FAM Dichlorotriazine

Background

Other Labeling Dyes

BioActs provides other traditionally used dyes such as 5(6)-Carboxyfluorescein (FAM) and 5(6)-Carboxytetramethylrhodamine (TAMRA) dyes for labeling of biomolecules. FAM is one of popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. In addition to relatively high absorptivity, good fluorescence quantum yield and good water solubility, FAM has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. TAMRA fluorophore has been a widely used for preparing bioconjugates, especially fluorescent antibody and avidin derivatives. TAMRA dye is also widely utilized for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore.

Other fluorescent dyes such as Cyanine, ICG, TAMRA, FAM, etc. are also available.
All dyes are equipped with various reactive and functional groups.
High quality and excellent performance

Table 1. FAM dye applications

Figure 1. Structure of FAM and TAMRA dyes

Citation & Reference

1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.

2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.

3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).

4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.

5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.