Flamma® 675 PEG4-Alkyne
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 675 PEG4-Alkyne
Cat. No. List below
Description
Flamma® Fluors 675 PEG4-alkyne is a sophisticated near-infrared (NIR) fluorescent dye designed for advanced bioimaging applications. This copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent is derived from a benzindocyanine structure, offering a stable and robust fluorescence signal ideal for various biological imaging techniques.
Key Features:
Spectral Properties - Excitation/Emission maxima: 675/691 nm
Spectrally similar to Alexa 680, Cy5.5, IRDye 680LT, and DyLight 680
Can be excited using a 633 nm laser line
Emits in the NIR region, allowing for deep tissue penetration
Its reactive group is a PEG4-alkyne, has a tetraethylene glycol spacer connecting the dye to the alkyne group and its molecular Weight is 1158.38 g/mol.
Flamma 675 PEG4-alkyne is particularly useful for cellular imaging, nucleotide functionalization, and click chemistry reactions in living systems. The dye's ability to form 1,4-disubstituted 1,2,3-triazoles through CuAAC reactions makes it an excellent choice for bioorthogonal labeling strategies. This reaction can occur within living systems without disrupting native biochemical processes.
To utilize Flamma 675 PEG4-alkyne effectively. introduce the azide functionality to the target biomolecule through chemical or genetic modification. Then perform the CuAAC reaction to couple the dye with the azide-modified target and finally proceed with imaging or further experimental procedures.
The NIR emission of Flamma 675 PEG4-alkyne makes it particularly valuable for in vivo imaging applications, as NIR light can penetrate deeper into biological tissues compared to visible light. This property allows for enhanced signal-to-noise ratios and improved imaging depth.
The PEG4 spacer not only provides improved water solubility but also helps to reduce non-specific binding, potentially leading to cleaner and more specific labeling in complex biological environments.
When using this dye for click chemistry applications, it's important to optimize reaction conditions, including copper catalyst concentration and reaction time, to ensure efficient labeling while minimizing potential cytotoxicity in live-cell experiments.
Flamma® Fluors 675 PEG4-alkyne represents a powerful tool in the arsenal of modern biomedical research, offering high sensitivity, specificity, and versatility for a wide range of imaging and labeling applications.
- Fluorophore: Flamma® Fluors 675
- Reactive group: PEG4-alkyne
- Excitation/Emission Max.(nm): 675/691
- Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT
- Extinction coefficient: ≥ 157,000 cm-1M-1
- CF280: 0.1
- Appearance: Blue Solid
- Molecular Weight: 1158.38 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWG1001 | Flamma® 496 PEG4-Alkyne | 496 | 520 | Alexa488, FITC, Cy2 |
| PWG1122 | Flamma® 552 PEG4-Alkyne | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWG1415 | Flamma® 581 PEG4-Alkyne | 581 | 596 | Alexa594, DyLight594 |
| PWG1215 | Flamma® 648 PEG4-Alkyne | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWG1515 | Flamma® 675 PEG4-Alkyne | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWG1301 | Flamma® 749 PEG4-Alkyne | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWG1603 | Flamma® 774 PEG4-Alkyne | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma 675 PEG4-alkyne offers numerous advantages over other near-infrared (NIR) dyes, making it a superior choice for bioimaging applications. Its excitation and emission maxima at 675/691 nm allow for optimal deep tissue penetration and reduced autofluorescence, enhancing imaging clarity. The dye's compatibility with a 633 nm laser line facilitates its use in various imaging systems. Additionally, the PEG4-alkyne reactive group enables efficient copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reactions, allowing for bioorthogonal labeling in living systems without disrupting native biochemical processes. With a high extinction coefficient of ≥ 157,000 cm?¹M?¹, Flamma 675 exhibits excellent brightness, while its tetraethylene glycol spacer improves solubility and minimizes non-specific binding. Furthermore, the dye maintains stability across a wide pH range and demonstrates high photostability, making it suitable for prolonged imaging sessions. Its spectral similarity to well-established dyes like Alexa 680 and Cy5.5 allows for easy integration into existing protocols, enabling effective multiplexing capabilities. Collectively, these features position Flamma 675 PEG4-alkyne as an exceptional tool for sensitive detection and advanced bioimaging applications.
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
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