SSPE (20X)
Cat. No. DCP-SSPE20X
Size: 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
20X SSPE (Saline-Sodium Phosphate-EDTA) is a concentrated buffer solution widely used in molecular biology, particularly for nucleic acid hybridization and blotting procedures. It serves as an essential component in Southern and Northern blotting techniques, acting as both a hybridization and transfer buffer. The inclusion of EDTA in SSPE is crucial as it chelates divalent metal ions like Mg²+, effectively inhibiting DNase activity and preserving the integrity of probe and target DNA during hybridization processes.The sodium phosphate in SSPE helps maintain an isotonic environment, which is particularly beneficial for RNA blotting. Additionally, 20X SSPE is commonly employed in microarray experiments as a washing buffer and in bacterial screening procedures. Its concentrated form allows for flexible dilution to various working concentrations, typically ranging from 0.1X to 20X, depending on the specific requirements of the experiment.
Application:
- Southern blotting: Used for DNA hybridization procedures.
- Northern blotting: Commonly employed in RNA hybridization and blotting techniques.
- Blot transfer: Facilitates the transfer of nucleic acids to membrane supports during blotting procedures.
- Microarray experiments: Serves as a washing buffer for DNA microarrays.
- Bacterial Screening: Utilized in bacterial screening procedures.
Advantages:
- Suitable for multiple applications, including Southern and Northern blotting, nucleic acid hybridizations, and microarray experiments.
- Can be diluted to various working concentrations (typically 0.1X to 20X) depending on specific experimental needs.
- Helps preserve the integrity of DNA and RNA during hybridization and transfer procedures.
- Provides an isotonic environment conducive to nucleic acid interactions.
Composition:
| Ingredients |
Concentration |
| Sodium Chloride |
3.0 M |
| Sodium Hydrogen Phosphate |
0.2 M |
| EDTA |
0.02 M |
Quality Control:
- Appearance: Clear solution.
- pH: 7.4
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
