Flamma® 648 Thiol
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 648 Thiol
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 648/663 nm
2. High extinction coefficient: ≥ 250,000 cm?¹M?¹
3. CF280: 0.03
4. Thiol functional group attached through a spacer
5. Blue solid appearance
6. Molecular weight: 743.99 g/mol
7. Soluble in DMF and DMSO
Applications:
1. Bioimaging and fluorescence microscopy
2. Labeling of biomolecules through disulfide bond formation
3. Reference standard for dye-conjugates
4. Super-resolution microscopy
5. Single-molecule detection
6. Fluorescence in situ hybridization (FISH)
7. Flow cytometry
8. Confocal laser scanning microscopy
9. Live-cell imaging
10. Deep-tissue and whole-body imaging
Advantages:
1. Operates in the optimal far-red spectrum, minimizing autofluorescence
2. Excellent signal-to-noise ratios
3. Spectral similarity to popular dyes (Alexa647, DyLight650, Cy5)
4. Efficient excitation using 593 or 633 nm laser lines
5. Versatile labeling options through disulfide bond formation with cysteine residues
6. Stable fluorescence signal for extended imaging sessions
7. High sensitivity for detecting low-abundance biological structures
8. Enhanced tissue penetration and reduced light scattering
9. Reduced phototoxicity to biological components
10. Compatibility with the "biological window" (650-900 nm) for improved in vivo imaging
Flamma® Fluors 648 Thiol features a unique structure where the thiol group is attached to the fluorophore through a spacer, enhancing its reactivity and flexibility in various experimental conditions. The dye's far-red spectral properties position it within the optimal "biological window," where light absorption by tissues is minimal, allowing for deeper penetration and improved signal-to-noise ratios in complex biological samples.
- Fluorophore: Flamma® Fluors 648
- Functional group: Thiol
- Excitation/Emission Max.(nm): 648/663
- Spectrally similar dyes: Alexa647, DyLight650, Cy5
- Extinction coefficient: ≥ 250,000 cm-1M-1
- CF280: 0.03
- Appearance: Blue Solid
- Molecular Weight: 743.99 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWT1001 | Flamma® 496 Thiol | 494 | 520 | Alexa488, FITC, Cy2 |
| CWT1058 | Flamma® 552 Thiol | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWT1415 | Flamma® 581 Thiol | 578 | 593 | Alexa594, DyLight594 |
| KWT1042 | Flamma® 648 Thiol | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWT1415 | Flamma® 675 Thiol | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWT1215 | Flamma® 749 Thiol | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWT1515 | Flamma® 774 Thiol | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Thiol
Flamma® Fluors thiol dyes have an attached primary thiol, which connected through a spacer. These thiol dyes can be labeled to biomolecules through disulfide bond formation with thiol of cysteine residue.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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