Flamma® 552 Hydrazide
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 552 Hydrazide
Cat. No. List below
Description
Flamma® Fluors 552 Hydrazide is a cutting-edge fluorescent dye designed for advanced bioimaging applications. This reactive bright yellow fluorophore, derived from a cyanine structure, offers exceptional stability in fluorescence signaling and is optimized for labeling biomolecules bearing aldehyde or ketone groups.
Key features:
1. Excitation/Emission maxima: 550/564 nm
2. High extinction coefficient: ≥ 136,000 cm?¹M?¹
3. Hydrazide reactive group for specific aldehyde and ketone labeling
4. Red solid appearance
5. Molecular weight: 686.88 g/mol
6. Soluble in DMF and DMSO
7. Spectrally similar to Alexa 555, DyLight 549, ATTO 550, and Cy3
Applications:
1. Labeling of polysaccharides and glycoproteins
2. Visualization of free reducing sugars on biomolecules
3. Bioimaging of aldehyde and ketone-bearing molecules
4. Potential use in flow cytometry and fluorescence microscopy
5. Applicable in studies involving carbohydrate chemistry and glycobiology
Advantages:
1. Generates stable fluorescence signals in bioimaging
2. Excellent optical properties when excited with a 488 nm laser line
3. Forms stable imine linkages through reductive amination reactions
4. Enables labeling of biomolecules after oxidation of primary and secondary alcohols
5. Offers compatibility with common fluorescence instrumentation
6. Provides a bright green fluorescence signal with minimal background
Specification
- Fluorophore: Flamma® Fluors 552
- Reactive group: Hydrazide
- Excitation/Emission Max.(nm): 550/564
- Spectrally similar dyes: Alexa555, DyLight549, Cy3, ATTO550
- Extinction coefficient: ≥ 136,000 cm-1M-1
- CF280: 0.07
- Appearance: Red Solid
- Molecular Weight: 686.88 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWH1001 | Flamma® 496 Hydrazide | 496 | 520 | Alexa488, FITC, Cy2 |
| PWH1122 | Flamma® 552 Hydrazide | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWH1415 | Flamma® 581 Hydrazide | 578 | 593 | Alexa594, DyLight594 |
| PWH1215 | Flamma® 648 Hydrazide | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWH1515 | Flamma® 675 Hydrazide | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWH1301 | Flamma® 749 Hydrazide | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWH1603 | Flamma® 774 Hydrazide | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Hydrazide
Flamma® Fluors hydrazide dyes can label aldehyde and ketone through reductive amination reaction to form an imine linkage. The main labeling target for hydrazides are free reducing sugars on biomolecules, and prior to conjugation, primary and secondary alcohols on polysaccharide and glycoprotein are usually oxidized to aldehyde and ketone. Fluorescent modification of aldehyde or carbonyl groups in carbohydrates is also frequently utilized for their analysis by HPLC, capillary electrophoresis and other methods. Hydrazide dyes can also label biomolecules, which introduced aldehyde by genetical or chemical modification.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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