Flamma® 774 Hydrazide
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 774 Hydrazide
Cat. No. List below
Description
Flamma® Fluors 774 Hydrazide exhibits specific reactivity towards carbonyl groups:
-Target Groups: Aldehydes and ketones
-Reaction Mechanism: Reductive amination to form an imine linkage
-Primary Targets: Free reducing sugars on biomolecules
-Pre-treatment: Oxidation of primary and secondary alcohols on polysaccharides and glycoproteins to aldehydes and ketones
Its applications include polysaccharide labeling (ideal for tagging complex carbohydrates), glycoprotein labeling (suitable for modifying glycosylated proteins), biomolecule detection (provides a stable fluorescence signal with high signal-to-noise ratio), and in vivo imaging (excellent for deep tissue imaging due to NIR properties).
The hydrazide group's specificity for carbonyls makes it particularly useful for glycobiology research and studies involving carbohydrate modifications. Its NIR properties make it excellent for applications requiring deep tissue penetration or where background fluorescence is a concern. The high signal-to-noise ratio enhances detection sensitivity in complex biological samples. Pre-oxidation of target molecules may be necessary to introduce carbonyl groups for efficient labeling.
Flamma® Fluors 774 Hydrazide represents a significant advancement in NIR fluorescent labeling technology, offering researchers a powerful tool for exploring complex biological systems with high specificity and sensitivity, particularly in the field of glycobiology and carbohydrate research.
What are the advantages?
1. High Sensitivity: The exceptionally high extinction coefficient (≥ 182,000 cm?¹M?¹) ensures strong signal generation
2. Low Protein Interference: The low CF280 value (0.1) minimizes interference with protein absorbance measurements
3. Versatility: Compatible with various imaging systems due to its spectral similarity to widely used NIR dyes
4. Stability: Generates stable fluorescence signals, ideal for long-term imaging studies
5. Specific Reactivity: Offers selective labeling of carbonyl-containing biomolecules
- Fluorophore: Flamma® Fluors 774
- Reactive group: Hydrazide
- Excitation/Emission Max.(nm): 774/800
- Spectrally similar dyes: Cy7.5, DyLight800, IRDye800CW
- Extinction coefficient: ≥ 182,000 cm-1M-1
- CF280: 0.1
- Appearance: Green Solid
- Molecular Weight: 943.09 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWH1001 | Flamma® 496 Hydrazide | 496 | 520 | Alexa488, FITC, Cy2 |
| PWH1122 | Flamma® 552 Hydrazide | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWH1415 | Flamma® 581 Hydrazide | 578 | 593 | Alexa594, DyLight594 |
| PWH1215 | Flamma® 648 Hydrazide | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWH1515 | Flamma® 675 Hydrazide | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWH1301 | Flamma® 749 Hydrazide | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWH1603 | Flamma® 774 Hydrazide | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Hydrazide
Flamma® Fluors hydrazide dyes can label aldehyde and ketone through reductive amination reaction to form an imine linkage. The main labeling target for hydrazides are free reducing sugars on biomolecules, and prior to conjugation, primary and secondary alcohols on polysaccharide and glycoprotein are usually oxidized to aldehyde and ketone. Fluorescent modification of aldehyde or carbonyl groups in carbohydrates is also frequently utilized for their analysis by HPLC, capillary electrophoresis and other methods. Hydrazide dyes can also label biomolecules, which introduced aldehyde by genetical or chemical modification.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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