qFlamma® Black01 NHS ester

qFlamma® Black01 NHS ester is a versatile, amine-reactive universal dark quencher with exceptional properties for various biological applications. This quencher exhibits a broad absorption range from 400 to 800 nm, with no native fluorescence, making it ideal for a wide array of fluorescent probes and assays.

Key Features
1. Broad Absorption Range: 400-800 nm, with a maximum at 490 nm
2. High Absorption Coefficient: 21,000 cm-1M-1
3. No Native Fluorescence: Ensures low background signal
4. Excellent Stability: Maintains performance in various conditions
5. High Aqueous Solubility: Facilitates use in biological systems
6. Reactive NHS Ester Group: Enables efficient conjugation to amine-containing biomolecules

Applications
1. Multiplexed Bio-analysis: Enables simultaneous detection of multiple targets
2. PCR and qPCR: Enhances sensitivity and specificity of nucleic acid amplification assays
3. Nucleic Acid Hybridization: Improves signal-to-noise ratio in hybridization-based techniques
4. FRET-based Assays: Acts as an efficient acceptor for a wide range of fluorophores
5. Custom-synthesized Activatable Probes: Allows design of sophisticated molecular probes
6. Protein and Peptide Labeling: Facilitates tracking and quantification of biomolecules

Advantages
1. Universal Quenching: Compatible with a wide range of fluorophores due to its broad absorption spectrum
2. High Sensitivity: Excellent quenching efficiency leads to improved signal-to-noise ratios
3. Versatility: Suitable for various biomolecules including nucleic acids, proteins, and peptides
4. Stability: Maintains performance in diverse experimental conditions
5. Water Solubility: Enables direct use in aqueous solutions without organic co-solvents
6. Efficient Conjugation: NHS ester group allows for rapid and specific labeling of amine-containing molecules
7. Multiplexing Capability: Broad absorption range supports simultaneous use of multiple fluorophores
8. Low Background: Absence of native fluorescence ensures minimal interference with target signals
9. Compatibility: Works well with common fluorescence-based techniques and instruments

qFlamma® Black01 NHS ester offers researchers a powerful tool for developing sensitive, specific, and versatile fluorescence-based assays across a wide range of biological applications. Its unique properties make it an excellent choice for both routine and advanced molecular biology techniques.

 

Specifications

• Functionality: Dark quencher
• Reactive group: NHS ester
• Absorption max. (nm): 490
• Absorption range (nm): 400 ~ 800
• Extinction coefficient: 21,000 cm-1M-1
• CF280: 0.8
• Appearance: Green Solid
• Molecular Weight: 818.83 g/mol
• Solubility: Water, DMF, DMSO
• Storage conditions: -20 ℃, protect from light

 qFlamma® Quenchers
 

Quick link (Cat.#)	Series	Quick link (Cat.#)	Series
QWS1001	qFlamma® Black01 NHS ester	QWSN1001	qFlamma® Black01 Sulfo-NHS ester
QWA1001	qFlamma® Black01 Vinylsulfone	QWC1001	qFlamma® Black01 Carboxylic acid
QWM1001	qFlamma® Black01 Maleimide	QWE1001	qFlamma® Black01 Amine
QWT1001	qFlamma® Black01 Thiol	QWR1001	qFlamma® Black01 Dichlorotriazine
QWG1001	qFlamma® Black01 PEG4-Alkyne	QWD1001	qFlamma® Black01 ADIBO
QWB1001	qFlamma® Black01 Biotin	QWK1001	qFlamma® Black01 Alkyne
QWZ1001	qFlamma® Black01 Azide	QWH1001	qFlamma® Black01 Hydrazide

qFlamma® Quenchers

Dark quenchers are substances that absorb excitation energy from a fluorophore and dissipate the energy as heat without any native fluorescence. The combination of a fluorescent dye and quencher may act as an on/off switch of fluorescence that the original fluorescence might be restored as the distance between them increases or when their connection is cleaved. In conjunction with fluorophores, dark quenchers are regarded as key components in designing FRET probes, in protease activity assays, nucleic acid hybridization, PCR, etc. The main advantage of dark quencher is the non-emissive excitation and elimination of background fluorescence. BioActs developed a new generation of azo-anthraquinone derivative dark quenchers, qFlamma® quenchers, to overcome issues associated with existing dark quenchers such as stability, quenching ability and water solubility.

The absorbance peaks of qFlamma® quenchers completely cover spectral range from visible to near-infrared (NIR) that all of commercially available fluorescent dyes can be effectively quenched. The quenchers display high absorption coefficient together with excellent quenching ability, superb stability and aqueous solubility so that it can easily manipulated in aqueous buffers. The four of qFlamma® quenchers (Black 01, Red, Blue and Orange) series cover the electromagnetic spectrum from 400 nm to NIR region and provides excellent spectral overlap across the entire range of known fluorescent dyes. The complete spectral overlap of qFlamma® quencher series along with their variety of reacting groups enable to design single-tube multiplexing assay system owing their capability for efficient quenching and the preventing cross-talking between dyes. BioActs offers qFlamma® quenchers as universal dark quenchers for the construction of a variety of hybridization and custom-synthesized probes incorporated spectrally paired fluorophores and quenchers for multiplexed bio-analysis, PCR, nucleic acid hybridization, and high-throughput imaging assays.