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Flamma® 648 ADIBO

Product#: DWC1021

$1,100.80

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Flamma® 648 ADIBO

Cat. No. List below

Description

Flamma® Fluors 648 ADIBO is a cutting-edge far-red fluorescent dye designed for strain-promoted azide-alkyne cycloaddition (SPAAC) reactions, offering unparalleled performance in bioimaging applications. This innovative reagent combines the power of copper-free click chemistry with exceptional fluorescence properties, providing researchers with a versatile and biocompatible tool for cellular imaging and biomolecule labeling.

Key Features and Advantages:

Optimal Spectral Properties: Excitation/emission maxima at 648/663 nm, comparable to popular dyes like Alexa 647, Cy5, and DyLight 650.
Versatile Excitation: Compatible with 593 or 633 nm laser lines, providing flexibility in imaging setups.
Superior Brightness: High extinction coefficient of ≥ 200,000 cm-1M-1 ensures vivid fluorescence signals.
Copper-Free Click Chemistry: ADIBO (azadibenzocyclooctyne) reactive group enables rapid, catalyst-free labeling.
Bioorthogonal Labeling: Forms 1,4-disubstituted 1,2,3-triazoles without disrupting native biochemical processes.
No Coupling Reagents Required: Simplifies labeling protocols and reduces potential toxicity

Flamma® Fluors 648 ADIBO excels in various applications, including live-cell imaging (ideal for real-time visualization of cellular processes without cytotoxicity concerns), In Vivo imaging (suitable for animal studies due to its biocompatibility and copper-free reaction), nucleotide functionalization (enables specific labeling of modified nucleic acids with high efficiency), protein labeling (excellent for tracking proteins in complex biological systems with minimal perturbation), and biomolecule tracking (perfect for studying dynamics of various biomolecules in living systems).

SPAAC Advantage:

The ADIBO reactive group allows for highly specific and efficient labeling through SPAAC reactions:

Bioorthogonal: Reactions occur without interfering with native biological processes.
Fast Kinetics: Rapid labeling even at low concentrations of reactants.
No Metal Catalysts: Eliminates potential toxicity associated with copper catalysts.
Mild Reaction Conditions: Compatible with a wide range of biological systems and pH values.

For best results, introduce azide functionality to target biomolecules via chemical or genetic modification, perform SPAAC reaction under physiological conditions without additional reagents, purify labeled biomolecules using standard chromatographic techniques if necessary and verify labeling efficiency through spectrophotometric analysis.

Specifications

Fluorophore: Flamma® Fluors 648
Reactive group: ADIBO
Excitation/Emission Max.(nm): 648/663
Spectrally similar dyes: Alexa647, DyLight650, Cy5
Extinction coefficient: ≥ 200,000 cm-1M-1
Appearance: Blue Solid
Molecular Weight: 943.18 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
DWC1001	Flamma® 496 ADIBO	496	520	Alexa488, FITC, Cy2
DWC1011	Flamma® 552 ADIBO	550	564	Alexa555, DyLight549, Cy3, ATTO550
DWC1415	Flamma® 581 ADIBO	581	596	Alexa594, DyLight594
DWC1021	Flamma® 648 ADIBO	648	663	Alexa647, DyLight650, Cy5
DWC1051	Flamma® 675 ADIBO	674	691	Alexa680, DyLight680, Cy5.5, IRDye680LT
DWC1031	Flamma® 749 ADIBO	749	774	Alexa750, DyLight755, Cy7.5, IRDye750
DWC1061	Flamma® 774 ADIBO	774	800	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

Why Choose Flamma® Fluors 648 ADIBO?

Cutting-Edge Technology: Combines copper-free click chemistry with far-red fluorescence for advanced bioimaging.
Exceptional Brightness: High extinction coefficient for clear, vivid signals even at low concentrations.
Biocompatibility: Ideal for live-cell and in vivo applications due to copper-free chemistry.
Versatility: Suitable for various biomolecules and imaging techniques.
Simplified Workflow: No need for copper catalysts or additional coupling reagents.

Flamma^® Fluors for Click Chemistry

The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma^® Fluors Alkyne dyes for CuAAC, Flamma^® Fluors ADIBO products for SPAAC and Flamma^® Fluors Azide dyes for both CuAAC and SPAAC.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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