Hep3B-miR122 Cell Line for HCV Propagation
Product#: FNK-20-001
$350.91
Availability:
Ships in 1-2 Weeks
Hep3B-miR122 Cell Line for HCV Propagation
Cat. No. FNK-20-001
Size 1 vial
Store -80°C
Description
Key words: Propagation of human Hepatitis C Virus, Cell culture-adapted HCV clone (HCVcc), miR122, Hepato cellular carcinoma line Hep3B.
Usage: Hep3B / miR122 cell line, a permissive cell line for the robust propagation of HCVcc by the expression of miR122 in Hep3B cells.
Cell line stock: 5 x 105 cells /1 ml in CELLBANKER-1 (cryopresevation media from Wako-Chemical, Osaka). Sent with dry-ice and store at -80℃
Growth medium: Dulbecco's modified Eagle's medium (DMEM) supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, and 10% fetal bovine serum (FBS).
Reference
Establishment and characterization of Hep3B/miR122 cell line has been described in the following publications.
- Kambara H, et al. (2012) Establishment of a novel permissive cell line for the propagation of hepatitis C virus by expression of microRNA miR122. J Virol. 86(3):1382-93. Open access.
- Review: Fukuhara T1, Matsuura Y. (2013). Role of miR-122 and lipid metabolism in HCV infection. J Gastroenterol. 48(2):169-76. Open access.
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