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Anti-HBsAg S, Polyclonal Antibody

Product#: FNK-BCL-ABPS-01

$497.06

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Anti-HBsAg S, Polyclonal Antibody

General information

Cat. No.	：FNK-FNK-BCL-ABPS-01
Size	：100µg
Source	：White rabbit
Appearance	：Liquid form（PBS containing biochemically inactive preservative）
Concentration	：1 mg/mL
Antigen	：HBsAg-S protein (genotype C, adr, BCL-AGSC-01）
Antigen specificity	：Bind only to Pre-S2 domain and not to S or Pre-S1domains
Purification	：Affinity by Protein A
Storage	：4°C (for long term storage at -20℃ avoid freeze and saw)
Usage	：ELISA (0.5 to 10 μg/mL)、Western blot (0.5 to 10 μg/mL)

Introduction

HBsAg is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle（diameter 50 to 60nm）in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection. Beacle has over a decade of R&D history on HBsAg and related materials. In this page we introduce HBsAg-related antibodies. All antibodies listed here are good for western blot and ELISA.

Description

Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. So called HBsAg is composed of either S-protein alone or a mixture of S- and M-proteins. The Pre-S1 region is known to be the hepatic cell recognition site and to be important in the HBV infection and Pre-S2 is reportedly known to related HBV entry or carcinogenesis. The present products are polyclonal antibody against S domain.

Anti-HBsAg-S, Polyclonal Antibody

Polyclonal antibody raised against HBs S-protein (so called HBsAg) in rabbit. The product can be used for western, and ELISA.

	Fig. 1: Genotype specificity The antibody bind to L-antigen of genotype A, B, C and D, but the relative affinity different depending on genotype as shown in the figure.
	Fig. 2: Western Blotting 1. Antibody: anti-Pre-S1（Code＃ BCL-ABP1-01） Antigen: BNC-L（Code＃ BCL-AG01） 2. Antibody: anti-Pre-S2（Code＃ BCL-ABP2-01） Antigen: BNC-L（Code＃ BCL-AG01） 3. Antibody: anti-S antigen（Code＃ BCL-ABPS-01） Antigen: BNC-XT（Code＃ BCL-AGX02） Two bands are seen in figures using either HBsAg-L or HBsAg-XT proteins as antigens. The upper band indicates dimer and the lower monomer.
	Fig. 3: ELISA conditon Immobilized IgG: anti-S antibody (polyclonal, BCL-ABPS-01) at 3 ug/mL Blocking: k-Block-e (Code#: BCL-BKHE-01) Detection IgG: anti-Pre-S2 antibody (monoclonal, HRP-labeled) at 20 ng/mL Antigen: HBsAg-XT (BCL-AGX-02)