Imidazole Buffer
Catalog No.: DCP-IMB
Size:
500 mL
Sterile: Filtered 0.1-micron membrane TWICE, 0.04-micron membrane TWICE
Storage: 2-8°C
Shelf Life: Unopened: 24 months
Opened: 8 weeks (tightly recap between uses)
*Use before the expiry date given on the product label.
pH: 7.35 ±0.05
Description:
A reagent fully compatible (not exclusive) with the Blood Coagulation Analyzer (Catalog No.: DCBio-SACA)
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A precisely formulated, ready-to-use aqueous buffer solution containing 0.05 mol/L (50 mM) imidazole and 0.1 mol/L (100 mM) sodium chloride, adjusted to pH 7.35 ± 0.2. Specifically designed as the standard diluent and vehicle (R2) in Thrombin Time (TT) assays, Clauss fibrinogen determinations, and coagulation factor activity assays, this buffer provides the physiologically compatible, low-ionic-strength aqueous environment necessary for accurate plasma dilution, thrombin stabilization, and reproducible clot endpoint detection. Compatible with manual, semi-automated, and fully automated optical and mechanical coagulation analyzers.
Why imidazole at this exact concentration: Imidazole's pKa of ~6.95 at 25°C (shifting to ~6.7 at 37°C) puts it squarely in the physiological buffering zone, and unlike bicarbonate, it maintains pH stability across the temperature transition from refrigerator to analyzer heating block. At 0.05 M with 0.1 M NaCl, ionic strength closely mimics plasma physiology without chelating calcium or inhibiting serine proteases like thrombin.
The pH 7.35 specification: Most commercial equivalents are formulated at pH 7.4; this product's pH 7.35 is within the standard ±0.2 tolerance and is analytically equivalent for TT and Clauss fibrinogen assays. The WHO NIBSC reference diluent specification for fibrinogen standardization is 0.05 M imidazole at pH 7.3–7.4, confirming this formulation's global conformance.
Composition:
| Ingredients |
| Imidazole |
| Sodium Chloride |
| Ultrapure Water |
* All DiagnoCine Precision Sterile filtered-sterilized with 40 nanometer. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
